Upton S J, Tilley M E, Marchin G L, Fina L R
Division of Biology, Kansas State University, Manhattan 66506.
J Parasitol. 1988 Aug;74(4):719-21.
The resin-I5 column developed at Kansas State University was tested for efficacy against oocysts of Cryptosporidium parvum (Apicomplexa: Cryptosporidiidae). Cesium chloride gradient-purified oocysts were passed through 1.0-cm-diameter columns with lengths of 2.5, 5.0, and 10.0 cm at 23 C. Following column passage, oocyst viability was determined both in vitro by excystation and in vivo by the ability to establish infections in suckling mice. Oocysts were found to be retained by the pentaiodide resin in a linear fashion, probably by electrostatic interactions. Linear regression analysis revealed 100% of the oocysts should be removed in such a manner using a column length of greater than or equal to 25.7 cm. When compared to untreated control oocysts, less than 12% of the oocysts that passed through the columns appeared to be affected by the resin, as assessed by excystation. Inoculation of suckling mice with these column-treated oocysts supported the excystation data and revealed the coccidian to be viable. These results indicate that oocysts of C. parvum are retained on the pentaiodide column in a 1-hit manner and that, although killing of parasites may occur within the column, the greatest effect that the column may have on the parasite is as an electrostatic retention device.
堪萨斯州立大学研发的树脂 - I5 柱针对微小隐孢子虫（顶复门：隐孢子虫科）的卵囊进行了功效测试。通过氯化铯梯度纯化的卵囊在 23℃下通过直径为 1.0 厘米、长度分别为 2.5 厘米、5.0 厘米和 10.0 厘米的柱子。柱通过后，通过体外脱囊和体内在乳鼠中建立感染的能力来确定卵囊活力。发现卵囊以线性方式被五碘化树脂保留，可能是通过静电相互作用。线性回归分析表明，使用长度大于或等于 25.7 厘米的柱子，应以这种方式去除 100%的卵囊。与未处理的对照卵囊相比，通过柱子的卵囊中，经脱囊评估，似乎只有不到 12%受到树脂影响。用这些经柱处理的卵囊接种乳鼠，证实了脱囊数据，并表明球虫是有活力的。这些结果表明，微小隐孢子虫的卵囊以单次打击的方式保留在五碘化柱上，并且尽管在柱内可能会发生寄生虫的杀灭，但该柱对寄生虫的最大作用可能是作为一种静电保留装置。
