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通过¹²⁵I表面标记鉴定三种隐孢子虫（顶复门：隐孢子虫科）的外卵囊壁蛋白

Identification of outer oocyst wall proteins of three Cryptosporidium (Apicomplexa: Cryptosporidiidae) species by 125I surface labeling.

作者信息

Tilley M, Upton S J, Blagburn B L, Anderson B C

机构信息

Division of Biology, Kansas State University, Manhattan 66506.

出版信息

Infect Immun. 1990 Jan;58(1):252-3. doi: 10.1128/iai.58.1.252-253.1990.

DOI:10.1128/iai.58.1.252-253.1990
PMID:2294053
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC258437/
Abstract

Autoradiography of oocyst wall surface proteins of three Cryptosporidium spp. revealed common bands at 285 to 290, 145 to 148, 120, 57, and 32 kilodaltons (kDa). Cryptosporidium baileyi and C. muris share proteins at 180, 100, 80 to 81, 29, and 18 to 19 kDa; C. baileyi and C. parvum share one protein at 46 to 47 kDa; and C. muris and C. parvum share a protein at 67 to 69 kDa. Additional protein bands, each unique to one species, were also observed.

摘要

三种隐孢子虫属卵囊壁表面蛋白的放射自显影显示，在285至290、145至148、120、57和32千道尔顿（kDa）处有共同条带。贝氏隐孢子虫和鼠隐孢子虫在180、100、80至81、29和18至19 kDa处有共享蛋白；贝氏隐孢子虫和微小隐孢子虫在46至47 kDa处有一个共享蛋白；鼠隐孢子虫和微小隐孢子虫在67至69 kDa处有一个共享蛋白。还观察到了每种仅在一个物种中出现的额外蛋白条带。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4412/258437/0c94a09a33b5/iai00049-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4412/258437/0c94a09a33b5/iai00049-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4412/258437/0c94a09a33b5/iai00049-0269-a.jpg

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本文引用的文献

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Cloning and sequence analysis of a highly polymorphic Cryptosporidium parvum gene encoding a 60-kilodalton glycoprotein and characterization of its 15- and 45-kilodalton zoite surface antigen products.编码一种60千道尔顿糖蛋白的微小隐孢子虫高度多态性基因的克隆与序列分析及其15千道尔顿和45千道尔顿子孢子表面抗原产物的特性研究
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Failure to differentiate Cryptosporidium parvum from C. meleagridis based on PCR amplification of eight DNA sequences.基于八个DNA序列的PCR扩增，无法区分微小隐孢子虫和火鸡隐孢子虫。
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