Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University (TMU), Tehran, Iran.
Department of Electrical and Computer Engineering, Tarbiat Modares University (TMU), Tehran, Iran.
Cell Biol Int. 2021 Sep;45(9):1851-1865. doi: 10.1002/cbin.11626. Epub 2021 May 20.
Dental tissue-derived stem cells (DSCs) provide an easy, accessible, relatively noninvasive promising source of adult stem cells (ASCs), which brought encouraging prospective for their clinical applications. DSCs provide a perfect opportunity to apply for a patient's own ASC, which poses a low risk of immune rejection. However, problems associated with the long-term culture of stem cells, including loss of proliferation and differentiation capacities, senescence, genetic instability, and the possibility of microbial contamination, make cell banking necessary. With the rapid development of advanced cryopreservation technology, various international DSC banks have been established for both research and clinical applications around the world. However, few studies have been published that provide step-by-step guidance on DSCs isolation and banking methods. The purpose of this review is to present protocols and technical details for all steps of cryopreserved DSCs, from donor selection, isolation, cryopreservation, to characterization and quality control. Here, the emphasis is on presenting practical principles in accordance with the available valid guidelines.
牙源性组织干细胞（DSCs）为成人干细胞（ASCs）提供了一种简便、可行、微创的来源，为其临床应用带来了广阔的前景。DSCs 为应用患者自身 ASC 提供了绝佳的机会，这降低了免疫排斥的风险。然而，干细胞长期培养相关的问题，包括增殖和分化能力的丧失、衰老、遗传不稳定性以及微生物污染的可能性，使得细胞库的建立成为必要。随着先进冷冻保存技术的快速发展，世界各地已经建立了各种用于研究和临床应用的国际 DSC 库。然而，关于 DSCs 分离和储存方法的分步指导，发表的研究很少。本综述的目的是介绍从供体选择、分离、冷冻保存到特性和质量控制的所有步骤中冷冻保存的 DSCs 的方案和技术细节。这里强调的是根据现有有效指南提出实用原则。
