Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
Department of Clinical Sciences, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden; Division of Obstetrics and Gynecology, Karolinska Universitetssjukhuset, Stockholm, Sweden; Ming Wai Lau Center for Reparative Medicine, Stockholm node, Karolinska Institutet, Stockholm, Sweden.
Stem Cell Reports. 2021 May 11;16(5):1117-1141. doi: 10.1016/j.stemcr.2021.03.019.
Detailed studies of the embryo allow an increasingly mechanistic understanding of development, which has proved of profound relevance to human disease. The last decade has seen in vitro cultured stem cell-based models of embryo development flourish, which provide an alternative to the embryo for accessible experimentation. However, the usefulness of any stem cell-based embryo model will be determined by how accurately it reflects in vivo embryonic development, and/or the extent to which it facilitates new discoveries. Stringent benchmarking of embryo models is thus an important consideration for this growing field. Here we provide an overview of means to evaluate both the properties of stem cells, the building blocks of most embryo models, as well as the usefulness of current and future in vitro embryo models.
对胚胎的详细研究使人们对发育的机制理解越来越深入，这对人类疾病的研究具有深远的意义。过去十年中，体外培养的基于干细胞的胚胎发育模型蓬勃发展，为可访问的实验提供了胚胎的替代物。然而，任何基于干细胞的胚胎模型的有用性都将取决于它在多大程度上准确地反映体内胚胎发育，以及它在多大程度上促进新的发现。因此，严格的胚胎模型基准测试是这个不断发展的领域的一个重要考虑因素。在这里，我们提供了一种评估干细胞特性的方法概述，这些干细胞是大多数胚胎模型的构建块，以及当前和未来的体外胚胎模型的有用性。
