Department of Biochemistry, Benjamin S. Carson (Snr.) School of Medicine, Babcock University, Ilishan-Remo, Ogun State, Nigeria.
Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.
J Complement Integr Med. 2021 May 12;19(1):19-26. doi: 10.1515/jcim-2019-0289.
The use of in folklore medicine for the management of reproductive disorders has been poorly reported. We sought to investigate the protective potential of the ethyl acetate fraction of stem bark extract (EFSC) on lead acetate-induced (LA) testicular toxicity in male rats.
Animals during a 28 days treatment received dimethyl sulfoxide (DMSO, 0.1%), LA (20 mg/kg), and EFSC (200 mg/kg). Others received EFSC only (100, 200, and 400 mg/kg) or vitamin E (100 mg/kg) 1 h prior to LA (20 mg/kg) administration.
LA administration decreased sperm counts and motility by 36.39 and 40.69% respectively in rats. Also, LA-untreated rats showed elevated malondialdehyde (MDA) and decreased total proteins in testis (260, 33%) and epididymis (62, 29%) respectively. However, EFSC (100, 200, or 400 mg/kg) administrations improved sperm morphological characteristics as well as antioxidant status in LA-treated rats. EFSC (400 mg/kg) showed improved testis seminiferous tubules that were almost normal in the LA-treated rats. Further, EFSC contains a high 9-octadecenoic acid methyl ester.
Overall, evidence by LA-induced testicular toxicity, EFSC provides chemopreventive roles via antioxidant mechanisms.
在民间医学中， 用于治疗生殖系统疾病的用途报道较少。我们旨在研究 茎皮提取物的乙酸乙酯部分（EFSC）对醋酸铅（LA）诱导的雄性大鼠睾丸毒性的保护潜力。
在 28 天的治疗期间，动物接受二甲基亚砜（DMSO，0.1%）、LA（20mg/kg）和 EFSC（200mg/kg）。其他动物则仅接受 EFSC（100、200 和 400mg/kg）或维生素 E（100mg/kg）1 小时，然后再给予 LA（20mg/kg）。
LA 给药使大鼠的精子计数和活力分别降低了 36.39%和 40.69%。此外，未经 LA 处理的大鼠睾丸和附睾中的丙二醛（MDA）水平升高，总蛋白含量分别降低了 260%（33%）和 62%（29%）。然而，EFSC（100、200 或 400mg/kg）给药改善了 LA 处理大鼠的精子形态特征和抗氧化状态。EFSC（400mg/kg）给药改善了 LA 处理大鼠的睾丸曲细精管，使其几乎恢复正常。此外，EFSC 含有高含量的 9-十八烯酸甲酯。
总体而言，LA 诱导的睾丸毒性表明 EFSC 通过抗氧化机制提供化学预防作用。
