Widders P R, Smith J W, Yarnall M, McGuire T C, Corbeil L B
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
J Med Microbiol. 1988 Aug;26(4):307-11. doi: 10.1099/00222615-26-4-307.
In-vitro culture of Haemophilus somnus in liquid or solid media supplemented with bovine blood or serum resulted in non-immune binding of immunoglobulin (Ig) by the organism. This binding was independent of the antigen-combining site of the Ig molecule, since binding of an IgG preparation specific for the hapten dinitrophenol was unaffected by the presence of the homologous antigen. Quantitative comparison of the binding of Ig fragments Fab and Fc demonstrated that the non-immune binding occurred in the Fc region of bovine IgG. The isotypes of Ig that became bound to H. somnus included both bovine IgG subclasses (IgG1 and IgG2), which were bound equally, and bovine IgM.
在添加牛血或血清的液体或固体培养基中对睡眠嗜血杆菌进行体外培养，结果发现该菌可与免疫球蛋白（Ig）发生非免疫性结合。这种结合与Ig分子的抗原结合位点无关，因为对二硝基苯酚半抗原具有特异性的IgG制剂的结合不受同源抗原存在的影响。对Ig片段Fab和Fc结合的定量比较表明，非免疫性结合发生在牛IgG的Fc区域。与睡眠嗜血杆菌结合的Ig同种型包括牛IgG亚类（IgG1和IgG2），二者结合程度相同，以及牛IgM。
