Zhao Likang, Li Tao, Chen Xiaorong, Lan Rongguang, Pang Zhen, Wu Sumei, Xiong Yanyun, Wu Yiqing, Wu Yougui, Lu Hongfei, Yu Mingjian
College of Life Science, Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
Qianjiangyuan-Baishan National Park Qinyuan Conservation Center, Qingyuan, 323800, China.
BMC Plant Biol. 2025 May 10;25(1):621. doi: 10.1186/s12870-025-06641-4.
Elevated temperatures significantly impaired the normal growth and development of plants. This study combined physiological and transcriptomic analyses to explore the potential mechanisms of response to heat stress in Abies beshanzuensis M. H. Wu. Under heat stress, A. beshanzuensis exhibited reduced photosynthetic rates and chlorophyll content, accompanied by marked downregulation of photosynthesis-associated genes, suggesting heat-induced photoinhibition and compromised carbon assimilation capacity. Furthermore, the increased activities of MDA, SOD, POD, and CAT suggested that A. beshanzuensis could withstand heat stress by enhancing the activity of antioxidant enzymes to mitigate excess reactive oxygen species and anions. Transcriptome analysis revealed the induction of genes related to heat shock proteins, plant hormone signaling, and antioxidants, which could enhance the tolerance of A. beshanzuensis to high temperatures. In summary, the research demonstrated that A. beshanzuensis could not tolerate high temperatures, which was identified as one of the primary reasons for its endangerment. This study offers a novel approach to investigating the regulatory mechanisms of heat stress.
温度升高显著损害了植物的正常生长和发育。本研究结合生理和转录组分析，探讨了百山祖冷杉对热胁迫响应的潜在机制。在热胁迫下，百山祖冷杉光合速率和叶绿素含量降低，同时光合作用相关基因显著下调，表明热诱导光抑制和碳同化能力受损。此外，丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性增加，表明百山祖冷杉可通过增强抗氧化酶活性来抵御热胁迫，以减轻过量的活性氧和阴离子。转录组分析揭示了与热休克蛋白、植物激素信号传导和抗氧化剂相关基因的诱导，这可以增强百山祖冷杉对高温的耐受性。总之，该研究表明百山祖冷杉不耐受高温，这被确定为其濒危的主要原因之一。本研究为研究热胁迫的调控机制提供了一种新方法。
