Vartak Richa, Patil Suyash M, Saraswat Aishwarya, Patki Manali, Kunda Nitesh K, Patel Ketan
College of Pharmacy & Health Sciences, St. John's University, New York, NY 11439, USA.
Nanomedicine (Lond). 2021 Jun;16(14):1187-1202. doi: 10.2217/nnm-2020-0475. Epub 2021 May 13.
To formulate an aerosolized nanoliposomal carrier for remdesivir (AL-Rem) against coronavirus disease 2019. AL-Rem was prepared using modified hydration technique. Cytotoxicity in lung adenocarcinoma cells, stability and aerodynamic characteristics of developed liposomes were evaluated. AL-Rem showed high encapsulation efficiency of 99.79%, with hydrodynamic diameter of 71.46 ± 1.35 nm and surface charge of -32 mV. AL-Rem demonstrated minimal cytotoxicity in A549 cells and retained monolayer integrity of Calu-3 cells. AL-Rem showed sustained release, with complete drug release obtained within 50 h in simulated lung fluid. Long-term stability indicated >90% drug recovery at 4°C. Desirable aerosol performance, with mass median aerodynamic diameter of 4.56 ± 0.55 and fine particle fraction of 74.40 ± 2.96%, confirmed successful nebulization of AL-Rem. AL-Rem represents an effective alternative for coronavirus disease 2019 treatment.
制备一种用于抗2019冠状病毒病的雾化纳米脂质体瑞德西韦载体（AL-Rem）。采用改良水合技术制备AL-Rem。评估了其对肺腺癌细胞的细胞毒性、所制备脂质体的稳定性和空气动力学特性。AL-Rem的包封率高达99.79%，流体动力学直径为71.46±1.35nm，表面电荷为-32mV。AL-Rem在A549细胞中表现出最小的细胞毒性，并保持了Calu-3细胞的单层完整性。AL-Rem显示出缓释特性，在模拟肺液中50小时内药物完全释放。长期稳定性表明在4°C时药物回收率>90%。理想的雾化性能，质量中值空气动力学直径为4.56±0.55，细颗粒分数为74.40±2.96%，证实了AL-Rem雾化成功。AL-Rem是治疗2019冠状病毒病的一种有效替代方案。
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