粘质沙雷氏菌对苏氨酸的降解作用。
Threonine degradation by Serratia marcescens.
作者信息
Komatsubara S, Murata K, Kisumi M, Chibata I
出版信息
J Bacteriol. 1978 Aug;135(2):318-23. doi: 10.1128/jb.135.2.318-323.1978.
Abstract
The wild strain of Serratia marcescens rapidly degraded threonine and formed aminoacetone in a medium containing glucose and urea. Extracts of this strain showed high threonine dehydrogenase and "biosynthetic" threonine deaminase activities, but no threonine aldolase activity. Threonine dehydrogenase-deficient strain Mu-910 was selected among mutants unable to grow on threonine as the carbon source. This strain did not form aminoacetone from threonine, but it slowly degraded threonine. Strain D-60, deficient in both threonine dehydrogenase and threonine deaminase, was derived from strain Mu-910 and barely degraded threonine. A glycine-requiring strain derived from the wild strain grew in minimal medium containing threonine as the glycine source, whereas a glycine-requiring strain derived from strain Mu-910 did not grow. This indicates that threonine dehydrogenase participates in glycine formation from threonine (via alpha-amino-beta-ketobutyrate) as well as in threonine degradation to aminoacetone.
摘要
粘质沙雷氏菌的野生菌株在含有葡萄糖和尿素的培养基中能迅速降解苏氨酸并生成氨基丙酮。该菌株的提取物显示出较高的苏氨酸脱氢酶和“生物合成”苏氨酸脱氨酶活性，但没有苏氨酸醛缩酶活性。在不能以苏氨酸作为碳源生长的突变体中筛选出了苏氨酸脱氢酶缺陷型菌株Mu - 910。该菌株不能从苏氨酸生成氨基丙酮，但能缓慢降解苏氨酸。菌株D - 60同时缺乏苏氨酸脱氢酶和苏氨酸脱氨酶，它由菌株Mu - 910衍生而来，几乎不能降解苏氨酸。从野生菌株衍生而来的甘氨酸需求型菌株能在以苏氨酸作为甘氨酸来源的基本培养基中生长，而从菌株Mu - 910衍生而来的甘氨酸需求型菌株则不能生长。这表明苏氨酸脱氢酶既参与从苏氨酸生成甘氨酸（通过α - 氨基 - β - 酮丁酸）的过程，也参与苏氨酸降解生成氨基丙酮的过程。
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