Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Anal Chem. 2021 May 25;93(20):7491-7498. doi: 10.1021/acs.analchem.1c00757. Epub 2021 May 13.
A novel multiplex electrochemiluminescence (ECL) polarization assay was developed to detect breast cancer-related genes BRCA1 and BRCA2 simultaneously based on the polarization characteristics of surface plasmon-coupled electrochemiluminescence (SPC-ECL). In this work, boron nitride quantum dots (BN QDs) were used as ECL emitters, and gold nanoparticles (Au NPs) and gold-coated silver nanoparticles (Ag@Au NPs) were employed as surface plasmon materials. The surface plasmon coupling resonance of different metal NPs not only enhanced the ECL intensity but also converted the isotropic emission into directional emission. This study revealed the relation between the structure of metal nanomaterials and SPC-ECL, and a high polarization-resolved sensing system was designed to detect multitarget DNA from 100 aM to 1 nM simultaneously. Polarization-based multiple ECL analysis has broad prospects in related cancer diagnosis and treatment evaluation.
一种新型的多重电化学发光（ECL）偏振分析方法是基于表面等离子体耦合电化学发光（SPC-ECL）的偏振特性，开发出来用于同时检测乳腺癌相关基因 BRCA1 和 BRCA2。在这项工作中，氮化硼量子点（BN QDs）被用作 ECL 发射器，金纳米粒子（Au NPs）和金包覆的银纳米粒子（Ag@Au NPs）被用作表面等离子体材料。不同金属 NPs 的表面等离子体耦合共振不仅增强了 ECL 强度，而且将各向同性发射转换为定向发射。本研究揭示了金属纳米材料的结构与 SPC-ECL 之间的关系，并设计了一个高偏振分辨传感系统，用于同时从 100 aM 到 1 nM 水平检测多种靶 DNA。基于偏振的多重 ECL 分析在相关癌症诊断和治疗评估方面具有广阔的前景。
