Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.
Anal Chem. 2023 Jul 4;95(26):9990-9998. doi: 10.1021/acs.analchem.3c01191. Epub 2023 Jun 23.
In this work, a novel plasmonic heterodimer with controllable hot spot was designed and applied to regulate surface plasmon coupling electrochemiluminescence (SPC-ECL) polarization sensing system. The heterodimer nanostructure consisted of individual Au-Ag core-shell nanocubes (Au@Ag NC) and Au nanospheres (Au NS), which were precisely assembled by thiol-DNA and biotin-streptavidin. The asymmetric nanostructure can significantly modulate the ECL intensity and emission polarization angle based on the synergy of the surface plasmon coupling (SPC) effect and the lightning rod effect with extraordinary field enhancement in the hot spot region. As a result, the isotropic ECL signal of zinc-doped nitrogen dots (Zn-N dots) was regulated in the directional emission. Furthermore, the SPC-ECL biosensor was successfully applied to detect miRNA-182 in triple-negative breast cancer (TNBC) tissues. The research on the established relationship between ECL polarization analysis and plasmonic heterodimers can provide a new pathway for the development of ECL sensing platforms.
在这项工作中，设计了一种具有可控热点的新型等离子体异质二聚体，并将其应用于调节表面等离子体耦合电化学发光（SPC-ECL）偏振传感系统。该异质二聚体纳米结构由单个 Au-Ag 核壳纳米立方体（Au@Ag NC）和 Au 纳米球（Au NS）组成，它们通过巯基-DNA 和生物素-链霉亲和素精确组装。不对称纳米结构可以基于表面等离子体耦合（SPC）效应和避雷针效应的协同作用，以及在热点区域的非凡场增强，显著调节 ECL 强度和发射偏振角。结果，锌掺杂氮点（Zn-N 点）的各向同性 ECL 信号被调节为定向发射。此外，成功地将 SPC-ECL 生物传感器应用于检测三阴性乳腺癌（TNBC）组织中的 miRNA-182。对 ECL 偏振分析与等离子体异质二聚体之间建立关系的研究可以为 ECL 传感平台的发展提供新途径。
