Department of Chemical Engineering and Materials Science, Graduate Program in System Health Science and Engineering, Ewha Womans University, Seoul, Republic of Korea.
Department of Biotechnology and Engineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University, Gwangju, Republic of Korea.
Biotechnol J. 2022 Mar;17(3):e2000451. doi: 10.1002/biot.202000451. Epub 2021 May 28.
The heavy global dependence on petroleum-based industries has led to serious environmental problems, including climate change and global warming. As a result, there have been calls for a paradigm shift towards the use of biorefineries, which employ natural and engineered microorganisms that can utilize various carbon sources from renewable resources as host strains for the carbon-neutral production of target products.
C4 alcohols are versatile chemicals that can be used directly as biofuels and bulk chemicals and in the production of value-added materials such as plastics, cosmetics, and pharmaceuticals. C4 alcohols can be effectively produced by microorganisms using DCEO biotechnology (tools to design, construct, evaluate, and optimize) and metabolic engineering strategies.
In this review, we summarize the production strategies and various synthetic tools available for the production of C4 alcohols and discuss the potential development of microbial cell factories, including the optimization of fermentation processes, that offer cost competitiveness and potential industrial commercialization.
全球对石油基产业的严重依赖导致了严重的环境问题，包括气候变化和全球变暖。因此，人们呼吁向使用生物精炼厂转变，生物精炼厂采用天然和工程微生物，可以利用可再生资源中的各种碳源作为宿主菌株，实现目标产物的碳中和生产。
C4 醇是一种多功能化学品，可直接用作生物燃料和大宗化学品，并可用于生产附加值材料，如塑料、化妆品和药品。微生物可以使用 DCEO 生物技术（设计、构建、评估和优化工具）和代谢工程策略有效地生产 C4 醇。
在这篇综述中，我们总结了生产 C4 醇的生产策略和各种合成工具，并讨论了微生物细胞工厂的潜在发展，包括发酵过程的优化，这提供了成本竞争力和潜在的工业商业化。
