Del Olmo Jon Andrade, Pérez-Álvarez Leyre, Pacha-Olivenza Miguel Ángel, Ruiz-Rubio Leire, Gartziandia Oihane, Vilas-Vilela José Luis, Alonso José Maria
Grupo de Química Macromolecular (LABQUIMAC), Departamento de Química Física, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, 48940 Leioa, Spain; i+Med S. Coop. Parque Tecnológico de Álava, Albert Einstein 15, nave 15, 01510 Vitoria-Gasteiz, Spain.
Grupo de Química Macromolecular (LABQUIMAC), Departamento de Química Física, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, 48940 Leioa, Spain; BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, Spain.
Int J Biol Macromol. 2021 Jul 31;183:1222-1235. doi: 10.1016/j.ijbiomac.2021.05.034. Epub 2021 May 10.
Bacterial contamination in implanted biomedical devices is a critical daily concern. The most used material for permanent implant in biomedical field is Ti6Al4V alloy due to its beneficial mechanical properties and high biocompatibility. Accordingly, in this work different polymeric antibacterial coatings poly(N-vinyl pyrrolidone) (PVP), hyaluronic acid (HA) and chitosan (CHI) were developed and comparatively analysed for Ti6Al4V surface covering. The adhesion of these coatings to Ti6Al4V substrates were carried out after the conjugation of these polymers with the so well-known bioadhesive properties of catechol (CA) anchor group. These surface modifications were characterized by X-ray photoelectronic spectroscopy, contact angle measurements and atomic force microscopy. In addition, the stability of CA-conjugated polymeric coatings was compared with the coatings formed with unconjugated polymers. Finally, the cytocompatibility and antibacterial properties against gram-positive and gram-negative strains on coated Ti6Al4V substrates were assessed confirming the effectiveness of these polymeric coatings against bacterial infections for future applications in protecting biomedical implants.
植入式生物医学设备中的细菌污染是日常面临的关键问题。由于其有益的机械性能和高生物相容性，Ti6Al4V合金是生物医学领域永久植入最常用的材料。因此，在本研究中，开发了不同的聚合物抗菌涂层，如聚N-乙烯基吡咯烷酮（PVP）、透明质酸（HA）和壳聚糖（CHI），并对Ti6Al4V表面覆盖情况进行了比较分析。在这些聚合物与具有众所周知的生物粘附特性的儿茶酚（CA）锚定基团共轭后，进行了这些涂层与Ti6Al4V基材的粘附。通过X射线光电子能谱、接触角测量和原子力显微镜对这些表面改性进行了表征。此外，还将CA共轭聚合物涂层的稳定性与未共轭聚合物形成的涂层进行了比较。最后，评估了涂覆Ti6Al4V基材上对革兰氏阳性和革兰氏阴性菌株的细胞相容性和抗菌性能，证实了这些聚合物涂层在保护生物医学植入物方面对细菌感染的有效性，可用于未来的应用。
