The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China.
College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China.
J Dairy Res. 2021 May;88(2):201-204. doi: 10.1017/S0022029921000455. Epub 2021 May 14.
We have previously bred Chinese local dairy sheep through grading up with local Small-Tailed Han (STH) sheep as female parent and DairyMeade (DM) sheep as male parent. In this research communication we characterize the whey protein profile of STH sheep and their offspring (F1, F2) to reveal physiological differences and variation in milk traits. A total of 1032 whey proteins were identified through tandem mass tag labeling (TMT) proteome profiling. Three proteins were significantly differentially abundant between F1 and STH milk, six between F2 and STH milk and five between F1 and F2 milk. In terms of differential changes between generations, WASHC4 and CUTA of F1 and Ig-like domain-containing protein of F2 milk were dominant whey proteins. Overall, the results showed that the whey protein profiles of different generations varied little. The crossbreeds of STH and DM sheep would be suitable for the development of the Chinese local sheep milk industry, and the F2 may be a better population for sheep milk production.
我们之前通过以本地小尾寒羊（STH）为母本和 DairyMeade（DM）羊为父本对中国本地奶绵羊进行了分级繁殖。在本研究通讯中，我们对 STH 绵羊及其后代（F1、F2）的乳清蛋白图谱进行了表征，以揭示生理差异和牛奶特性的变化。通过串联质量标签标记（TMT）蛋白质组谱分析鉴定了 1032 种乳清蛋白。F1 和 STH 奶之间有 3 种蛋白质丰度差异显著，F2 和 STH 奶之间有 6 种，F1 和 F2 奶之间有 5 种。就代际间的差异变化而言，F1 的 WASHC4 和 CUTA 以及 F2 的 Ig 样结构域蛋白是主要的乳清蛋白。总的来说，结果表明不同代际的乳清蛋白图谱变化不大。小尾寒羊和 DM 羊的杂交品种适合中国本地绵羊奶产业的发展，F2 可能是更好的绵羊奶生产种群。
