The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China.
College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China.
J Dairy Res. 2021 Nov;88(4):420-424. doi: 10.1017/S002202992100073X. Epub 2021 Dec 6.
We characterized the proteome profile of mid-lactation small-tailed Han (STH) and DairyMeade (DM) ovine milk in order to explore physiological variation and differences in milk traits between the two breeds. Methodology combined a tandem mass tag (TMT) proteomic approach with LC-MS/MS technology. A total of 656 proteins were identified in STH and DM ovine milk, of which 17and 29 proteins were significantly upregulated (P < 0.05) in STH and DM, respectively. Immune-related proteins and disease-related proteins were highly expressed in STH milk, whereas S100A2 and AEBP1 were highly expressed in DM milk, which had beneficial effects on mammary gland development and milk yield. Our results provide a theoretical basis for future breeding of dairy sheep.
本研究旨在探究两种绵羊品种——小尾寒羊（STH）和 DairyMeade（DM）——泌乳中期乳蛋白组特征，从而探索品种间乳特性的生理差异。本研究采用串联质量标签（TMT）蛋白质组学方法与 LC-MS/MS 技术相结合。共鉴定出 STH 和 DM 绵羊乳中的 656 种蛋白质，其中 STH 和 DM 中分别有 17 种和 29 种蛋白质显著上调（P < 0.05）。STH 乳中高度表达免疫相关蛋白和疾病相关蛋白，而 S100A2 和 AEBP1 在 DM 乳中高度表达，这对乳腺发育和产奶量有有益影响。本研究结果为未来奶绵羊的选育提供了理论基础。
