Cho Sung Hyun, Lee Seung-Sook, Manjula Prabuddha, Kim Minjun, Lee Seung Hwan, Lee Jun Heon, Seo Dongwon
Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea.
Jisan Farm, Nonsan 29009, Korea.
J Anim Sci Technol. 2020 Nov;62(6):790-800. doi: 10.5187/jast.2020.62.6.790. Epub 2020 Nov 30.
The Yeonsan Ogye (YO) chicken is a natural heritage of Korea, characterized by black feathers, skin, bones, eyes, and comb. The purebred of YO population has been reared under the natural mating system with no systematic selection and breeding plan. The purpose of this study was to identify the genetic diversity and find the optimal number of population sub-division using 12 polymorphic microsatellite (MS) markers to construct a pedigree-based breeding plan for the YO population. A total of 509 YO birds were used for this study. Genetic diversity and population structure analysis were conducted based on the MS marker genotype information. The overall average polymorphic information content value and expected heterozygosity of the population were 0.586, and 0.642, respectively. The K-mean cluster analysis based on the genetic distance result confirmed that the current YO population can be divided into three ancestry groups. Individuals in each group were evaluated based on their genetic distance to identify the potential candidates for a future breeding plan. This study concludes that a future breeding plan with known pedigree information of selected founder animals, which holds high genetic diversity, could be the best strategy to ensure the conservation of the Korean YO chicken population.
岭南五黑鸡是韩国的自然遗产，其特征是羽毛、皮肤、骨骼、眼睛和鸡冠均为黑色。岭南五黑鸡种群的纯种一直是在自然交配系统下饲养的，没有系统的选择和育种计划。本研究的目的是利用12个多态微卫星（MS）标记来鉴定遗传多样性并找出种群细分的最佳数量，从而为岭南五黑鸡种群构建基于系谱的育种计划。本研究共使用了509只岭南五黑鸡。基于MS标记基因型信息进行了遗传多样性和种群结构分析。该种群的总体平均多态信息含量值和期望杂合度分别为0.586和0.642。基于遗传距离结果的K均值聚类分析证实，当前的岭南五黑鸡种群可分为三个祖先群体。根据每组个体的遗传距离进行评估，以确定未来育种计划的潜在候选个体。本研究得出结论，拥有已知系谱信息且具有高遗传多样性的选定奠基动物的未来育种计划可能是确保韩国岭南五黑鸡种群得以保护的最佳策略。
