Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
Network Center for Animal Breeding and Omics Research, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
Trop Anim Health Prod. 2021 May 14;53(2):321. doi: 10.1007/s11250-021-02664-9.
Plasma membrane lipids are the key factor in the ability of chicken sperm to be frozen. They ensure fluidity and flexibility of the sperm membrane for effective viability and motility during in vitro storage. The objective of this study was to compare the lipid profiles of different native and commercial chicken breeds: native Thai (Pradu Hang Dam) roosters and commercial (Rhode Island Red) roosters, with respect to their frozen sperm quality. In addition, lipid peroxidation and antioxidant enzymes superoxidase dismutase (SOD) and catalase (CAT) were also examined. Semen was collected from 12 roosters of each breed. For fresh semen, parameters assessed include semen volume, pH, sperm concentration, sperm motility, and viability, while for frozen semen, the parameters assessed were sperm motility and viability. Moreover, other parameters assessed included malondialdehyde (MDA) concentration, activities of SOD and CAT, and fatty acid profile. We found that sperm viability and motility of frozen semen were higher in the commercial breed than in the native breed (P < 0.05). The commercial chicken breed had higher MDA concentration than the native breed (P < 0.05), but antioxidant enzymes remained unchanged in both. Levels of arachidonic acid (AA; C20:4n-6) and docosahexaenoic acid (DHA; C22:6n-3) were significantly higher (P < 0.05) in the commercial than in the native breed; however, n-6 to n-3 ratios were not different. In conclusion, our study found that lipid profiles have an influence on frozen sperm viability and motility between the breeds. Polyunsaturated fatty acids, particularly AA and DHA, are beneficial to sperm quality.
质膜脂质是鸡精子冷冻能力的关键因素。它们确保了精子膜的流动性和柔韧性，从而在体外储存期间提高了精子的活力和运动能力。本研究的目的是比较不同本地和商业鸡品种（泰国本地鸡（Pradu Hang Dam）公鸡和商业（罗得岛红）公鸡）的脂质谱，以研究其冷冻精子质量。此外，还检查了脂质过氧化和抗氧化酶超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。从每个品种的 12 只公鸡中采集精液。对于新鲜精液，评估的参数包括精液量、pH 值、精子浓度、精子活力和活力，而对于冷冻精液，评估的参数包括精子活力和活力。此外，还评估了其他参数，包括丙二醛（MDA）浓度、SOD 和 CAT 的活性以及脂肪酸谱。我们发现，商业品种的冷冻精液精子活力和活力高于本地品种（P < 0.05）。商业鸡种的 MDA 浓度高于本地鸡种（P < 0.05），但两种鸡种的抗氧化酶活性均保持不变。花生四烯酸（AA；C20:4n-6）和二十二碳六烯酸（DHA；C22:6n-3）的水平在商业品种中明显高于本地品种（P < 0.05）；然而，n-6 到 n-3 的比值没有差异。总之，我们的研究发现，脂质谱对品种间的冷冻精子活力和活力有影响。多不饱和脂肪酸，特别是 AA 和 DHA，对精子质量有益。
