Aniento F, Garcia-España A, Portolés M, Alonso E, Cabo J R
Departamento de Bioquimica, Facultad de Farmacia, Universidad de Valencia, Spain.
Mol Cell Biochem. 1988 Feb;79(2):107-12. doi: 10.1007/BF02424551.
Canaline and gabaculine, inhibitors of gamma-aminotransferases and thus of ornithine aminotransferase (E.C. 2.6.1.13), decreased the flow through ornithine carbamoyl transferase (E.C. 2.1.3.3) in isolated rat hepatocytes incubated with 10 mM NH4Cl and ornithine. The levels of acetylglutamate, an essential activator of carbamoyl phosphate synthetase (ammonia) (E.C. 6.3.4.16), were also decreased, suggesting that the inhibitors had also caused a decrease in the rate of carbamoyl phosphate synthesis. Under these conditions, ornithine appears to be a precursor of acetylglutamate, via ornithine aminotransferase, possibly as a consequence of glutamate synthesis. The influence of aminooxyacetate, an aminotransferase inhibitor, has also been examined.
犬尿氨酸和加巴喷丁，作为γ-氨基转移酶抑制剂，因而也是鸟氨酸氨基转移酶（E.C. 2.6.1.13）的抑制剂，在与10 mM氯化铵和鸟氨酸一起孵育的离体大鼠肝细胞中，降低了通过鸟氨酸氨甲酰基转移酶（E.C. 2.1.3.3）的通量。氨甲酰磷酸合成酶（氨）（E.C. 6.3.4.16）的必需激活剂乙酰谷氨酸水平也降低，这表明抑制剂也导致了氨甲酰磷酸合成速率的下降。在这些条件下，鸟氨酸似乎通过鸟氨酸氨基转移酶成为乙酰谷氨酸的前体，这可能是谷氨酸合成的结果。还研究了氨基氧基乙酸（一种氨基转移酶抑制剂）的影响。
