Kam P L, Lin C C, Li J C, Meng C L, Chang G G
Department of Biochemistry, National Defense Medical Center, Taiwan, Republic of China.
Mol Cell Biochem. 1988 Feb;79(2):171-9. doi: 10.1007/BF02424560.
Rabbit antibodies against pigeon liver malic enzyme (EC 1.1.1.40) were prepared. The antiserum gave single precipitation line with crude pigeon liver extract. Cross reaction was observed with partially purified malic enzyme or crude extract from chicken liver. Positive cross reaction was also observed with the concentrated cytosolic fraction of two human carcinoma cell lines which were demonstrated to contain high malic enzyme activity. All other proteins examined did not react with the antibodies. When purified pigeon liver malic enzyme was mixed with the antiserum in vitro, a time-dependent inactivation of the enzyme activity was observed. Protection of the enzyme activity against antiserum inactivation was afforded by NADP+ or L-malate. Metal Mn2+ gave little protection.
制备了抗鸽肝苹果酸酶（EC 1.1.1.40）的兔抗体。该抗血清与粗制鸽肝提取物产生单一沉淀线。观察到与部分纯化的苹果酸酶或鸡肝粗提取物有交叉反应。在两种人癌细胞系的浓缩胞质部分也观察到阳性交叉反应，这两种细胞系被证明含有高苹果酸酶活性。所有检测的其他蛋白质均不与抗体反应。当在体外将纯化的鸽肝苹果酸酶与抗血清混合时，观察到酶活性随时间的失活。NADP + 或L-苹果酸可保护酶活性免受抗血清失活的影响。金属Mn2 + 的保护作用很小。
