Povey S, Jeremiah S, Arthur E, Ber R, Fialkow P J, Gardiner E, Goodfellow P N, Karande A, Klein G, Quintero M, Steel C M, Zeuthen J
Ann Hum Genet. 1981 Jul;45(3):237-52. doi: 10.1111/j.1469-1809.1981.tb00335.x.
Soluble malic enzyme (MEs) has been examined in long-term human lymphoid cell lines cultured from 101 individuals. In 65 out of 66 lines derived from people without lymphoreticular malignancy the enzyme was very active. Lines established from 35 individuals with various forms of lymphoreticular malignancy were also examined, including in some cases more than 1 line derived from the same patient. In all cases where the cell line was thought to be derived from normal cells MEs was active, but in 27 out of 29 lines thought to be derived from malignant cells (from 25 patients) MEs was not detected. In the case of two patients with chronic lymphatic leukaemia 'normal' lines active for malic enzyme, and 'leukaemic' lines lacking malic enzyme, had been cultured from the same individual. Preliminary investigations of the lack of malic enzyme in somatic cell hybrids derived from lymphoma and leukaemia cell lines are compatible with an alteration at the level of the structural locus MEs on chromosome 6. However, the restoration of MEs activity in one line by fusion with mouse teratocarcinoma cells suggests that the alteration may be of a regulatory nature.
已对从101名个体培养的长期人类淋巴细胞系中的可溶性苹果酸酶（MEs）进行了检测。在从无淋巴网状恶性肿瘤的人衍生的66个细胞系中的65个中，该酶非常活跃。还检测了从35名患有各种形式淋巴网状恶性肿瘤的个体建立的细胞系，在某些情况下，同一患者衍生出不止一个细胞系。在所有认为细胞系源自正常细胞的情况下，MEs都是活跃的，但在认为源自恶性细胞（来自25名患者）的29个细胞系中的27个中未检测到MEs。在两名慢性淋巴细胞白血病患者中，已从同一个体培养出了对苹果酸酶有活性的“正常”细胞系和缺乏苹果酸酶的“白血病”细胞系。对源自淋巴瘤和白血病细胞系的体细胞杂种中缺乏苹果酸酶的初步研究表明，这与6号染色体上MEs结构基因座水平的改变相符。然而，通过与小鼠畸胎瘤细胞融合，一个细胞系中MEs活性的恢复表明这种改变可能是调节性的。
