The Talent Culturing Plan for Leading Disciplines of Shandong Province, Department of Chemistry and Chemical Engineering, Jining University, Qufu, 273155, Shandong, China.
Department of Chemistry, Bohai University, Jinzhou, 121013, Liaoning, China.
Anal Bioanal Chem. 2021 Jul;413(16):4255-4265. doi: 10.1007/s00216-021-03376-7. Epub 2021 May 14.
Anderson-type polyoxometalate containing Fe and Mo, (NH)[HFe(III)MoO] (FeMo), was found to work as an oxidase-mimicking nanoenzyme for the first time, exhibiting the ability of catalytic oxidation of o-phenylenediamine (OPD), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTs), and 3,3',5,5'-tetramethylbenzidine (TMB), which features easy synthesis, low cost, simple operation, and low consumption. Attributed to the nature of FeMo and Fenton-like effect, a novel sensor based on two consecutive "turn on" fluorescence was developed for detecting dopamine (DA) by employing the FeMo-OPD system, and the linear range was from 1 to 100 μM with the detection limit 0.0227 μM (3σ/s). Moreover, to increase oxidase-mimic activity of FeMo, reduced graphene oxide (rGO) loading FeMo composites (FeMo@rGO (n), n = 5%, 10%, 15%) was fabricated, and results show that oxidase-like activities of FeMo@rGO (n) are dependent on the mass ratio of FeMo/rGO, and FeMo@rGO (10%) exhibits the highest oxidase-mimic activity and the fastest respond time (4 min) among all reported oxidase mimic of DA to date. Graphical abstract Anderson-type Mo-POMs FeMo was found to work as an oxidase-mimicking nanoenzyme for the first time and was used to detect DA for two consecutive "turn on" fluorescence sensor modes.
Anderson 型多金属氧酸盐（POMs）含有 Fe 和 Mo，（NH）[HFe（III）MoO]（FeMo），首次被发现具有氧化酶模拟纳米酶的作用，能够催化氧化邻苯二胺（OPD）、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和 3,3',5,5'-四甲基联苯胺（TMB），具有合成简单、成本低、操作简单、消耗低等特点。由于 FeMo 的特性和类 Fenton 效应，采用 FeMo-OPD 体系，开发了一种基于两次连续“开启”荧光的新型传感器，用于检测多巴胺（DA），线性范围为 1 至 100 μM，检测限为 0.0227 μM（3σ/s）。此外，为了提高 FeMo 的氧化酶模拟活性，制备了负载还原氧化石墨烯（rGO）的 FeMo 复合材料（FeMo@rGO（n），n=5%、10%、15%），结果表明，FeMo@rGO（n）的类氧化酶活性依赖于 FeMo/rGO 的质量比，在所有报道的 DA 氧化酶模拟物中，FeMo@rGO（10%）表现出最高的类氧化酶模拟活性和最快的响应时间（4 分钟）。
