College of Veterinary Medicine, Hebei Agricultural University, Baoding, China.
Henan Institute of Veterinary Drug and Feed Control, Zhengzhou Henan, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2021 Jul;38(7):1136-1147. doi: 10.1080/19440049.2021.1914868. Epub 2021 May 14.
In this study, molecularly imprinted microspheres of a type capable of recognising amantadine and rimantadine were first synthesised, and three fluorescent tracers based on dansyl chloride, fluorescein isothiocyanate and 5-carboxytetramethylrhodamine were also synthesised. These reagents were used to develop and optimise a direct competitive fluorescence method on conventional 96-well microplate for detection of the two analytes. Results showed that this method achieved simple operation procedure, rapid assay process (30 min), high sensitivity (limits of detection 0.04-0.05 ng mL) and acceptable recycle performance (five times). After optimisation of several parameters, this method was used to detect amantadine and rimantadine in chicken muscle samples. Their recoveries from standards fortified blank samples were in the range of 62.3-93.7%. The analysis results for some real chicken samples were consistent with a confirmatory LC-MS/MS method. Therefore, this method could be used as a rapid, simple and accurate tool for routine screening the residues of amantadine and rimantadine in a large number of chicken muscle samples.
在这项研究中，首次合成了能够识别金刚烷胺和金刚乙胺的分子印迹微球，并合成了三种基于丹磺酰氯、异硫氰酸荧光素和 5-羧基四甲基罗丹明的荧光示踪剂。这些试剂被用于开发和优化传统 96 孔微板上的直接竞争荧光法，以检测这两种分析物。结果表明，该方法具有操作简单、分析快速（30 分钟）、灵敏度高（检测限为 0.04-0.05ng/mL）和可接受的循环性能（五次）等特点。在优化了几个参数后，该方法被用于检测鸡肉样品中的金刚烷胺和金刚乙胺。从标准添加空白样品中回收的回收率在 62.3-93.7%之间。一些实际鸡肉样品的分析结果与确证的 LC-MS/MS 方法一致。因此，该方法可作为一种快速、简单、准确的工具，用于常规筛选大量鸡肉样品中金刚烷胺和金刚乙胺的残留。
