College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China.
College of Science, Hebei Agricultural University, Baoding, Hebei 071000, China.
Food Chem. 2020 Jul 30;319:126539. doi: 10.1016/j.foodchem.2020.126539. Epub 2020 Mar 2.
Two molecularly imprinted microspheres and two fluorescent tracers for benzimidazoles and pyrethroids were synthesized respectively. The two types of microspheres were coated in the wells of conventional microplate simultaneously. Then the sample extracts and the two traces were added for differential competition. The fluorescence intensities at two different emission wavelengths were excited and recorded for quantification of the two classes of drugs respectively. The optimized multiplexed fluorescence method could be used to determine 8 benzimidazoles and 10 pyrethroids in mutton and beef samples simultaneously. The limits of detection of the method for the 18 drugs were in the range of 5.2-17 ng/mL, and the recoveries from the standards fortified blank samples were in the range of 67.7%-109%. From the analysis of 60 real mutton and beef samples, this method could be used for multi-screening the residues of benzimidazoles and pyrethroids in meat samples.
分别合成了两种用于苯并咪唑类和拟除虫菊酯类的分子印迹微球和两种荧光示踪剂。将这两种微球同时包被在常规微孔板的孔中。然后加入样品提取物和两种示踪剂进行差异竞争。分别在两个不同的发射波长下激发和记录荧光强度，以定量两种药物。优化的多指标荧光法可同时测定羊肉和牛肉样品中的 8 种苯并咪唑类和 10 种拟除虫菊酯类药物。该方法对 18 种药物的检出限在 5.2-17 ng/mL 范围内，从标准添加空白样品中回收的范围在 67.7%-109%之间。通过对 60 份实际羊肉和牛肉样品的分析，该方法可用于多筛查肉样中苯并咪唑类和拟除虫菊酯类药物的残留。
