Department of Marine Biology, Faculty of Marine Sciences and Technology, University of Hormozgan, Bandar Abbas, Iran.
Department of Marine Biology, Faculty of Marine Sciences and Technology, University of Hormozgan, Bandar Abbas, Iran.
Int J Biol Macromol. 2017 Nov;104(Pt A):883-888. doi: 10.1016/j.ijbiomac.2017.06.099. Epub 2017 Jun 27.
Chitin extraction from shrimp wastes by biological treatment, using the Pseudomonas aeruginosa was a positive and simple method. In order to look for the optimal conditions, the wastes were incubated at 30°C and 100rpm in different glucose (0%, 10%, 15% and 20%) and inoculation (10%, 15% and 20%) concentrations for 4 and 6days. At the end of fermentation, Protease activity was investigated at different temperatures and temperature 50°C was considered as the optimum. The results obtained also showed a direct relationship between the concentration of different parameters and deproteinization and demineralization rates, so that the optimal conditions were 20% glucose, 20% inoculation and 6days fermentation. These conditions led to 82% demineralization, 92% deproteinization and chitin yield of 47%. Then, chitin was converted to chitosan using microwave, autoclave and traditional methods. The highest yield (87%) was obtained with autoclave method. At the end, the chitin and chitosan were characterized by elemental analysis and FTIR.
利用铜绿假单胞菌对虾废料进行生物处理提取甲壳素是一种积极而简单的方法。为了寻找最佳条件，将废料在 30°C 和 100rpm 下，在不同的葡萄糖（0％，10％，15％和 20％）和接种（10％，15％和 20％）浓度下孵育 4 天和 6 天。在发酵结束时，在不同温度下研究蛋白酶活性，并且将 50°C 的温度视为最佳温度。所得结果还表明，不同参数的浓度与脱蛋白和脱矿质率之间存在直接关系，因此最佳条件为 20％葡萄糖，20％接种和 6 天发酵。这些条件导致脱矿质率为 82％，脱蛋白率为 92％，甲壳素得率为 47％。然后，使用微波，高压灭菌器和传统方法将甲壳素转化为壳聚糖。高压灭菌器法获得的产率最高（87％）。最后，通过元素分析和 FTIR 对甲壳素和壳聚糖进行了表征。
