Qinghai University, Qinghai Academy of Agricultural and Forestry Sciences, Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining, Qinghai Province, 810016, China.
BMC Plant Biol. 2024 Jul 12;24(1):666. doi: 10.1186/s12870-024-05334-8.
Hulless barley (Hordeum vulgare L. var. nudum Hook. f.) is a significant cereal crop and a substantial source of forage for livestock. Long non-coding RNAs (lncRNAs) and metabolites play crucial roles in the nutrient accumulation and regeneration of hulless barley plants following mowing. The study aimed to identify differentially expressed lncRNAs and metabolites in hulless barley plants by analyzing transcriptomic and metabolomic datasets at 2 h, 24 h, and 72 h following mowing.
The study revealed that 190, 90, and 438 lncRNA genes were differentially expressed at the 2 h, 24 h, and 72 h time points compared to the non-mowing control. We identified 14 lncRNA genes-11 downregulated and 3 upregulated-showing consistently significant differential expression across all time points after mowing. These differentially expressed lncRNAs target genes involved in critical processes such as cytokinin signaling, cell wall degradation, storage protein accumulation, and biomass increase. In addition, we identified ten differentially expressed metabolites targeting diverse metabolic pathways, including plant hormones, alkaloids, and flavonoids, before and after mowing at various time points. Endogenous hormone analysis revealed that cytokinin most likely played a crucial role in the regeneration of hulless barley after mowing.
This study created a comprehensive dataset of lncRNAs, metabolites, and hormones in hulless barley after mowing, revealing valuable insights into the functional characteristics of lncRNAs, metabolites, and hormones in regulating plant regeneration. The results indicated that cytokinin plays a significant role in facilitating the regeneration process of hulless barley after mowing. This comprehensive dataset is an invaluable resource for better understanding the complex mechanisms that underlie plant regeneration, with significant implications for crop improvement.
裸大麦（Hordeum vulgare L. var. nudum Hook. f.）是一种重要的谷类作物，也是牲畜的重要饲料来源。长链非编码 RNA（lncRNA）和代谢物在刈割后裸大麦植株的养分积累和再生中起着至关重要的作用。本研究旨在通过分析刈割后 2 h、24 h 和 72 h 的转录组和代谢组数据集，鉴定裸大麦中差异表达的 lncRNA 和代谢物。
研究表明，与未刈割对照相比，在 2 h、24 h 和 72 h 时，有 190、90 和 438 个 lncRNA 基因差异表达。我们鉴定了 14 个 lncRNA 基因，其中 11 个下调，3 个上调，在刈割后所有时间点均表现出一致的显著差异表达。这些差异表达的 lncRNA 靶向参与细胞分裂素信号转导、细胞壁降解、贮藏蛋白积累和生物量增加等关键过程的基因。此外，我们还鉴定了在刈割前后不同时间点的 10 种差异表达代谢物，这些代谢物靶向多种代谢途径，包括植物激素、生物碱和类黄酮。内源激素分析表明，细胞分裂素很可能在裸大麦刈割后再生过程中发挥关键作用。
本研究创建了裸大麦刈割后 lncRNA、代谢物和激素的综合数据集，揭示了 lncRNA、代谢物和激素在调节植物再生功能特征方面的有价值的信息。结果表明，细胞分裂素在促进裸大麦刈割后再生过程中起着重要作用。这个综合数据集是更好地理解植物再生背后复杂机制的宝贵资源，对作物改良具有重要意义。
