School of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan Province 421001, PR China.
School of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan Province 421001, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Oct 5;259:119897. doi: 10.1016/j.saa.2021.119897. Epub 2021 May 4.
A facile, friendly and one-step hydrothermal protocol was used to synthesize nitrogen-doped carbon dots (N-CDs) by utilizing hexamethylenetetramine and ethanediamine as the carbon and nitrogen sources. It demonstrated good water solubility and fluorescence properties were stable, whether in acidic or alkaline. Quantum yield (QY) of N-CDs was 8.3% at an excitation wavelength of 325 nm with maximum emission at 425 nm. The fluorescence of N-CDs achieved very high fluorescence quenching of 60% in the detection of p-nitrophenol (p-NP) in aqueous medium via fluorescence resonance energy transfer (FRET) mechanisms. Under optimum conditions, fluorescence probs of N-CDs had strong selectivity to p-NP, and the fluorescence intensity was linearly proportional to p-NP concentration from 0.5 to 70.0 μM with a detection limit of 0.201 μM. The corresponding cell experiments were also performed, indicating that the prepared N-CDs possessed low cytotoxicity and good biocompatibility. Meanwhile, the N-CDs can be used for the determination of p-NP in river water and industrial wastewater.
一种简便、友好的一步水热法，利用六亚甲基四胺和乙二胺作为碳源和氮源合成了氮掺杂碳点（N-CDs）。它具有良好的水溶性，荧光性质稳定，无论是在酸性还是碱性条件下。在激发波长为 325nm 时，N-CDs 的量子产率（QY）为 8.3%，最大发射波长为 425nm。N-CDs 的荧光通过荧光共振能量转移（FRET）机制，在水介质中对对硝基苯酚（p-NP）的检测实现了高达 60%的荧光猝灭。在最佳条件下，N-CDs 的荧光探针对 p-NP 具有很强的选择性，荧光强度与 p-NP 浓度在 0.5 至 70.0 μM 范围内呈线性关系，检测限为 0.201 μM。相应的细胞实验也表明，所制备的 N-CDs 具有低细胞毒性和良好的生物相容性。同时，N-CDs 可用于测定河水和工业废水中的 p-NP。
