State Key Laboratory of Marine Environmental Science, Fujian Key Laboratory of Marine Carbon Sequestration, Xiamen University, Xiamen, China.
Curr Microbiol. 2021 Jul;78(7):2815-2820. doi: 10.1007/s00284-021-02519-4. Epub 2021 May 15.
A Gram-stain-negative, strictly aerobic, yellow-pigmented bacterial strain, designated as T28, was isolated from seawater of the shallow-sea hydrothermal system, Kueishantao Islet, Taiwan, China. Cells were oxidase-negative and catalase-positive rods without gliding motility. Growth was observed at 10-40 °C (optimum, 30 °C), at pH 5.0-8.0 (optimum, pH 6.0) and in the presence of 0-5% (w/v) NaCl (optimum, 2.5%). Strain T28 contained menaquinone 6 as the only isoprenoid quinone. The main cellular fatty acids were iso-C, iso-C G, and iso-C3-OH, summed feature 3 (Cω7c/ω6c). Polar lipids contain diphosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, six unidentified lipids, an unidentified aminolipid, and one unidentified aminophospholipids. The genomic DNA G + C content was 34.4 mol%. The 16S rRNA gene sequence of strain T28 shared highest similarity with Maribacter arcticus (97.7%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain T28 belongs to the genus Maribacter. On the basis of phylogenetic data and several distinct phenotypic characteristics, strain T28 represents a novel species, for which the name Maribacter hydrothermalis sp. nov. is proposed. The type strain is T28 (=CGMCC 1.15788 = JCM 31510).
一株革兰氏阴性、严格需氧、呈黄色的细菌菌株，被命名为 T28，从中国台湾省龟山岛浅海热液系统的海水中分离得到。细胞呈氧化酶阴性、过氧化氢酶阳性的杆状，无滑动运动。在 10-40°C（最佳温度 30°C）、pH5.0-8.0（最佳 pH6.0）和 0-5%（w/v）NaCl（最佳 2.5%）存在下可生长。菌株 T28 含有menaquinone 6 作为唯一的异戊二烯醌。主要细胞脂肪酸为 iso-C、iso-C G 和 iso-C3-OH，特征性脂肪酸为 summed feature 3 (Cω7c/ω6c)。极性脂含有双磷脂酰甘油、磷脂酰乙醇胺、两种未鉴定的磷脂、六种未鉴定的脂类、一种未鉴定的胺脂和一种未鉴定的氨基磷脂。基因组 DNA G+C 含量为 34.4 mol%。菌株 T28 的 16S rRNA 基因序列与北极海 Maribacter arcticus（97.7%）的相似度最高。基于 16S rRNA 基因序列的系统发育分析表明，该菌株属于 Maribacter 属。基于系统发育数据和几个明显的表型特征，菌株 T28 代表一个新种，命名为 Maribacter hydrothermalis sp. nov.。模式菌株为 T28（=CGMCC 1.15788=JCM 31510）。
