State Key Laboratory of Marine Environmental Science, Fujian Key Laboratory of Marine Carbon Sequestration, Xiamen University, Xiamen, PR China.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6251-6256. doi: 10.1099/ijsem.0.004525. Epub 2020 Oct 19.
A novel Gram-stain-negative, non-flagellated, non-motile, rod-shaped (0.4-0.6×1.8-2.5 µm), aerobic bacterial strain, designated JLT21, was isolated from seawater of a shallow-sea hydrothermal system. Growth occurred with 0-4.0 % (w/v) NaCl (optimum, 2.0 % NaCl), at 8-45 °C (optimum, 25 °C) and at pH 3.0-10.0 (optimum, pH 7.0). Analysis of 16S rRNA gene sequences revealed that strain JLT21 showed the highest 16S rRNA gene sequence similarity to KEM-8 (97.2 %), IMCC1914 (96.9 %) and GW1-1 (96.9 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JLT21 clustered with GW1-1. The predominant respiratory quinone of strain JLT21 was menaquinone-6 (MK-6). Polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, three unidentified glycolipids and four unidentified lipids. The major fatty acids of strain JLT21 were iso-C (21.7 %), C 6/7 (11.5 %) and iso-C 3-OH (10.9 %). The DNA G+C content of strain JLT21 was 32.6 %. On the basis of polyphasic analysis, strain JLT21 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is JLT21 (=CGMCC 1.15787=JCM 31511). The study helps us better understand the bacterial species in the shallow-sea hydrothermal system and their adaptations to the hydrothermal environment.
一株新型革兰氏阴性、无鞭毛、不运动、杆状（0.4-0.6×1.8-2.5μm）、需氧细菌菌株，被命名为 JLT21，从浅海热液系统的海水中分离得到。该菌在 0-4.0%（w/v）NaCl（最佳，2.0%NaCl）、8-45℃（最佳，25℃）和 pH3.0-10.0（最佳，pH7.0）的条件下生长。16S rRNA 基因序列分析表明，菌株 JLT21 与 KEM-8（97.2%）、IMCC1914（96.9%）和 GW1-1（96.9%）的 16S rRNA 基因序列具有最高的相似性。基于 16S rRNA 基因序列的系统发育分析表明，菌株 JLT21 与 GW1-1 聚为一簇。菌株 JLT21 的主要呼吸醌为menaquinone-6（MK-6）。极性脂包括磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺、三种未鉴定的糖脂和四种未鉴定的脂类。菌株 JLT21 的主要脂肪酸为 iso-C（21.7%）、C 6/7（11.5%）和 iso-C 3-OH（10.9%）。菌株 JLT21 的 DNA G+C 含量为 32.6%。基于多相分析，菌株 JLT21 代表一个新的属种，建议命名为 sp. nov.。该种的模式菌株为 JLT21（=CGMCC 1.15787=JCM 31511）。该研究有助于我们更好地了解浅海热液系统中的细菌物种及其对热液环境的适应。
