Department of Food Engineering, Post-Graduate Program in Food Science and Technology, Technology Center, Federal University of Paraiba, João Pessoa, PB, Brazil; Department of Molecular Biology, Laboratory of Structural Proteomics, Federal University of Paraíba, João Pessoa, PB, Brazil.
Faculty of Health Sciences of Trari, Federal University of Rio Grande do Norte, Santa Cruz, RN, Brazil.
Food Res Int. 2021 May;143:110286. doi: 10.1016/j.foodres.2021.110286. Epub 2021 Mar 9.
Bioactive peptides have been broadly studied for their contribution to human health. This study aimed to identify bioactive peptides generated by in vitro gastrointestinal digestion of yam proteins. Yam protein concentrate (YPC) was submitted to simulated digestion. Gastric phase hydrolysate (GPH) and total gastrointestinal phase hydrolysate (GIPH) had their peptides identified by nanoLC-ESI-MS/MS. Peptide sequences were subjected to a database-driven (BIOPEP) bioactivity search. In vitro tests included: Antioxidant activity, DNA damage protection, ACE-inhibitory activity and antibacterial activity against the bacteria Escherichia coli, Salmonella sp. and Lysteria monocytogenes. Simulated digestion generated small peptides (mostly MW < 3500 Da), several of them with potential bioactive sequences predicted in silico. In both GPH and GIPH biological activities were detected, although GIPH displayed stronger DNA damage protection and antibacterial activity against Escherichia coli. The digestion of yam proteins releases promising biologically active peptides which can contribute to the prevention of bacterial infection and chronic degenerative diseases, with beneficial effects to human health.
生物活性肽因其对人类健康的贡献而被广泛研究。本研究旨在鉴定山药蛋白体外胃肠消化生成的生物活性肽。山药蛋白浓缩物（YPC）经模拟消化处理。胃阶段水解产物（GPH）和总胃肠阶段水解产物（GIPH）的肽段通过纳升电喷雾串联质谱（nanoLC-ESI-MS/MS）进行鉴定。肽序列通过数据库驱动（BIOPEP）进行生物活性搜索。体外试验包括：抗氧化活性、DNA 损伤保护、ACE 抑制活性和对大肠杆菌、沙门氏菌和单核细胞增生李斯特菌的抗菌活性。模拟消化生成了小肽（主要 MW<3500 Da），其中一些在计算机中预测具有潜在生物活性序列。在 GPH 和 GIPH 中均检测到了生物活性，尽管 GIPH 对大肠杆菌的 DNA 损伤保护和抗菌活性更强。山药蛋白的消化释放出有前途的生物活性肽，有助于预防细菌感染和慢性退行性疾病，对人类健康有益。
