Instituto de Lactología Industrial (INLAIN, UNL-CONICET), Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe, Argentina.
Laboratorio de Plancton, Instituto Nacional de Limnología (INALI, UNL-CONICET), Universidad Nacional del Litoral, Santa Fe, Argentina.
Food Res Int. 2020 Nov;137:109673. doi: 10.1016/j.foodres.2020.109673. Epub 2020 Sep 17.
Obesity and overweight, and their concomitant metabolic diseases, emerge as one of the most severe health problems in the world. Prevention and management of obesity are proposed to begin early in childhood, when probiotics may have a role. The Simulator of the Human Intestinal Microbial Ecosystem (SHIME®), in a dynamic validated in vitro system able to simulate the different parts of the gastrointestinal tract, has proven to be useful in analyzing the human intestinal microbial community. L. plantarum 73a and B. animalis subsp. lactis INL1, two strains isolated from breast milk, were assayed in the SHIME® using the fecal microbiota of an obese child. L. plantarum 73a alone or in combination with B. animalis subsp. lactis INL1 demonstrated survival capacity in the SHIME® system. The administration of both strains increased the alpha diversity of the microbiota and reduced the levels of the phylum Proteobacteria. In particular, the genera Escherichia, Shigella, and Clostridium_sensu_stricto_1 were significantly reduced when both strains were administered. The increase of Proteobacteria phylum is generally associated with the microbiota of obese people. Escherichia and Shigellacan be involved in inflammation-dependent adiposity and insulin resistance. L. plantarum73a supplementation reduced ammonia production. L. plantarum 73a alone or in combination with B. animalis subsp. lactis INL1 are potential probiotic candidates for the management of infant obesity.
肥胖症和超重，以及由此产生的代谢性疾病，是世界上最严重的健康问题之一。预防和管理肥胖症被提议在儿童早期开始，此时益生菌可能发挥作用。人类肠道微生物生态系统模拟器（SHIME®）是一种动态的、经过验证的体外系统，能够模拟胃肠道的不同部位，已被证明在分析人类肠道微生物群落方面非常有用。从母乳中分离出的两株菌，植物乳杆菌 73a 和乳双歧杆菌亚种 lactis INL1，在 SHIME®中使用肥胖儿童的粪便微生物群进行了检测。植物乳杆菌 73a 单独或与乳双歧杆菌亚种 lactis INL1 联合使用，在 SHIME®系统中具有生存能力。两种菌株的给药增加了微生物群的 alpha 多样性，并降低了厚壁菌门的水平。特别是，当两种菌株给药时，属大肠杆菌、志贺菌和严格梭菌 1 的数量显著减少。厚壁菌门的增加通常与肥胖人群的微生物群有关。大肠杆菌和志贺菌可能与炎症依赖性肥胖和胰岛素抵抗有关。植物乳杆菌 73a 的补充减少了氨的产生。植物乳杆菌 73a 单独或与乳双歧杆菌亚种 lactis INL1 联合使用可能是管理婴儿肥胖的潜在益生菌候选物。
