Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, South Korea.
Department of Research and Development, Mjbiogen corp., Seoul 04788, South Korea.
Ecotoxicol Environ Saf. 2021 Aug;219:112318. doi: 10.1016/j.ecoenv.2021.112318. Epub 2021 May 13.
Curcumin exhibited numerous key activities including antioxidant, anti-inflammatory, and immunostimulatory effects in fish. This study evaluated the protective effects of curcumin (CUR) against lead (Pb)-induced toxicities in fish. Healthy Cyprinus carpio L. individuals were segregated into control, Pb only, Pb+CUR, and CUR only groups. Pb groups were exposed to 1 mg L of Pb, and CUR groups were fed a basal diet supplemented with 15 g kg of CUR. After eight weeks, growth performance, Pb accumulation in tissues, various haemato-biochemical parameters, immune responses, and cytokine gene expression were measured. Dietary CUR effectively decreased Pb accumulation in tissues and increased the survival of Pb-exposed fish. Co-treatment with Pb and CUR reversed alterations in haemato-biochemical parameters, ameliorated Pb-induced oxidative stress, enhanced immune responses, and restored intestinal enzymatic activities. Dietary CUR reversed changes in intestinal microbiota in Pb-exposed fish. Pb-induced upregulation of NF-κBp65 and HSP70 was inhibited by dietary CUR. CUR supplementation upregulated the mRNA levels of SOD, Nrf2, IL-10, and CYP450 1A and attenuated Pb-induced degradation of I κB-α mRNA levels. Overall, CUR antagonizes Pb-induced negative impacts in fish. Thus, dietary CUR had several beneficial effects on immune responses, decreased Pb accumulation in tissues, and reversed Pb-induced oxidative stress in fish. Therefore, CUR plays a protective role in Pb-induced immune toxicity in fish, and, as such, may be suitable as an aqua feed additive for use in carp aquaculture.
姜黄素在鱼类中表现出许多关键活性，包括抗氧化、抗炎和免疫刺激作用。本研究评估了姜黄素（CUR）对鱼类铅（Pb）诱导毒性的保护作用。将健康的鲤鱼个体分为对照组、Pb 组、Pb+CUR 组和 CUR 组。Pb 组暴露于 1mg/L 的 Pb，CUR 组喂食基础饲料，补充 15g/kg 的 CUR。八周后，测量生长性能、组织中 Pb 积累、各种血液生化参数、免疫反应和细胞因子基因表达。膳食 CUR 有效降低了组织中的 Pb 积累，提高了 Pb 暴露鱼类的存活率。Pb 和 CUR 共同处理逆转了血液生化参数的改变，改善了 Pb 诱导的氧化应激，增强了免疫反应，并恢复了肠道酶活性。膳食 CUR 逆转了 Pb 暴露鱼类肠道微生物群的变化。Pb 诱导的 NF-κBp65 和 HSP70 上调被膳食 CUR 抑制。CUR 补充上调了 SOD、Nrf2、IL-10 和 CYP450 1A 的 mRNA 水平，并减弱了 Pb 诱导的 IκB-α mRNA 水平的降解。总的来说，CUR 拮抗了 Pb 对鱼类的负面影响。因此，膳食 CUR 对免疫反应有多种有益影响，减少了组织中 Pb 的积累，并逆转了 Pb 诱导的鱼类氧化应激。因此，CUR 在鱼类 Pb 诱导的免疫毒性中发挥保护作用，因此可能适合作为鲤鱼养殖中的水产饲料添加剂。
