Ridsdale J A, Rattner J B, Davie J R
Department of Biochemistry, University of Manitoba, Winnipeg, Canada.
Nucleic Acids Res. 1988 Jul 11;16(13):5915-26. doi: 10.1093/nar/16.13.5915.
The chromatin of several genes was assayed for sensitivity to DNAase I and for solubility as polynucleosomes in 0.15 M NaCl. The degree of solubility of chromatin fragments as polynucleosomes in 0.15 M NaCl correlates well with the sensitivity to DNAase I for several genes. Chromatin of repressed, housekeeping and erythroid-specific genes can be distinguished as distinct groups by the degree to which they display these properties. NaCl precipitation of chromatin fragments stripped and then reconstituted with varying quantities of H1 and H5 (linker) histones indicate that the polynucleosomes of erythroid-specific genes have altered interaction with these histones. Linker histones interacted with bulk chromatin and in the chromatin of the repressed ovalbumin and vitellogenin genes to form salt precipitable structures. Chromatin of erythroid-specific genes (histone H5 and beta-globin) as well as that of the histone H2A.F gene was resistant to linker histone induced precipitation.
对几个基因的染色质进行了检测，分析其对脱氧核糖核酸酶I的敏感性以及在0.15M氯化钠中作为多聚核小体的溶解性。染色质片段在0.15M氯化钠中作为多聚核小体的溶解程度与几个基因对脱氧核糖核酸酶I的敏感性密切相关。通过抑制基因、管家基因和红系特异性基因显示这些特性的程度，可以将它们的染色质区分为不同的组。对染色质片段进行氯化钠沉淀，去除后再用不同量的H1和H5（连接）组蛋白进行重构，结果表明红系特异性基因的多聚核小体与这些组蛋白的相互作用发生了改变。连接组蛋白与整体染色质以及抑制的卵清蛋白和卵黄生成素基因的染色质相互作用，形成盐沉淀性结构。红系特异性基因（组蛋白H5和β-珠蛋白）以及组蛋白H2A.F基因的染色质对连接组蛋白诱导的沉淀具有抗性。
Zotero 插件
