在接近生理条件下，组蛋白H1可以从鸡红细胞染色质中被选择性去除。
Histone H1 can be removed selectively from chicken erythrocyte chromatin at near physiological conditions.
作者信息
Muyldermans S, Lasters I, Wyns L
出版信息
Nucleic Acids Res. 1980 Feb 25;8(4):731-9.
Abstract
Histone H1 was depleted selectively from chicken erythrocyte polynucleosomes, without any detectable concomitant loss of H5 or core particle histones. The depletion is performed with ion exchange resin at low ionic strength (80 mM NaCl). The nucleosomes did not slide during the procedure. In contrast to the native chromatin, H1 depleted polynucleosomes are completely soluble in the 5--600 mM NaCl range.
摘要
组蛋白H1从鸡红细胞多核小体中被选择性去除，而H5或核心颗粒组蛋白没有任何可检测到的伴随损失。这种去除是在低离子强度（80 mM NaCl）下用离子交换树脂进行的。在此过程中核小体没有滑动。与天然染色质不同，H1缺失的多核小体在5 - 600 mM NaCl范围内完全可溶。
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