Kaidbey K
Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia.
Photodermatol. 1988 Apr;5(2):65-70.
Exposure of mammalian skin to ultraviolet radiation (UVR) results in a transient inhibition of scheduled DNA synthesis. The wavelength dependence for this response was investigated in the epidermis of albino hairless mice. Groups of animals were exposed to narrow wavebands of UVR (HPBW 6.6 nm) from a monochromator in the 260-335 nm range. A dose-dependent inhibition of DNA synthesis was observed following exposure to all test wavebands except that centered at 335 nm. An action spectrum constructed from dose-response regression lines showed peak effectiveness at 290 nm. This spectrum bears a close resemblance to published action spectra for the induction of pyrimidine dimers in vivo, suggesting that DNA is the primary chromophore for both events. The DNA synthesis inhibition spectrum bears little resemblance to a published therapeutic action spectrum for the clearing of psoriasis.
哺乳动物皮肤暴露于紫外线辐射（UVR）会导致预定DNA合成的短暂抑制。在白化无毛小鼠的表皮中研究了这种反应的波长依赖性。将动物分组，使其暴露于单色仪发出的260 - 335 nm范围内的窄波段UVR（半高宽6.6 nm）。除了以335 nm为中心的波段外，在暴露于所有测试波段后均观察到DNA合成的剂量依赖性抑制。根据剂量 - 反应回归线构建的作用光谱在290 nm处显示出最大有效性。该光谱与已发表的体内嘧啶二聚体诱导作用光谱非常相似，表明DNA是这两个事件的主要发色团。DNA合成抑制光谱与已发表的银屑病清除治疗作用光谱几乎没有相似之处。
