Malit Jessie James Limlingan, Wu Chuanhai, Liu Ling-Li, Qian Pei-Yuan
Department of Ocean Science and Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory, The Hong Kong University of Science and Technology, Hong Kong, China.
Shaanxi Key Laboratory of Natural Products and Chemical Biology, College of Chemistry and Pharmacy, Northwest A&F University, Yangling, China.
Front Microbiol. 2021 Apr 30;12:635389. doi: 10.3389/fmicb.2021.635389. eCollection 2021.
Thioamidated ribosomally synthesized and post-translationally modified peptides (RiPPs) are recently characterized natural products with wide range of potent bioactivities, such as antibiotic, antiproliferative, and cytotoxic activities. These peptides are distinguished by the presence of thioamide bonds in the peptide backbone catalyzed by the YcaO-TfuA protein pair with its genes adjacent to each other. Genome mining has facilitated an approach to identify biosynthesis gene clusters (BGCs) responsible for thioamidated RiPP production. In this work, publicly available genomic data was used to detect and illustrate the diversity of putative BGCs encoding for thioamidated RiPPs. AntiSMASH and RiPPER analysis identified 613 unique TfuA-related gene cluster families (GCFs) and 797 precursor peptide families, even on phyla where the presence of these clusters have not been previously described. Several additional biosynthesis genes are colocalized with the detected BGCs, suggesting an array of possible chemical modifications. This study shows that thioamidated RiPPs occupy a widely unexplored chemical landscape.
硫代酰胺化核糖体合成及翻译后修饰肽（RiPPs）是最近被鉴定的天然产物，具有广泛的强效生物活性，如抗生素、抗增殖和细胞毒性活性。这些肽的特征在于肽主链中存在硫代酰胺键，该键由YcaO - TfuA蛋白对催化，其基因彼此相邻。基因组挖掘促进了一种识别负责硫代酰胺化RiPPs产生的生物合成基因簇（BGCs）的方法。在这项工作中，公开可用的基因组数据被用于检测和说明编码硫代酰胺化RiPPs的假定BGCs的多样性。AntiSMASH和RiPPER分析确定了613个独特的与TfuA相关的基因簇家族（GCFs）和797个前体肽家族，即使在以前未描述过这些簇存在的门中也是如此。几个额外的生物合成基因与检测到的BGCs共定位，表明存在一系列可能的化学修饰。这项研究表明，硫代酰胺化RiPPs占据了一个广泛未被探索的化学领域。
