Boly Rainatou, Yabre Zakaline, Nitiema Mathieu, Yaro Boubacar, Yoda Jules, Belemnaba Lazare, Ilboudo Sylvain, Youl Noëla Hoho Estelle, Guissou Innocent Pierre, Ouedraogo Sylvin
Institute of Research in Health Science (IRSS), 03 PO 7047, Ouagadougou 03, Burkina Faso.
Laboratory of Drug Development, Doctoral School of Sciences and Health, University Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso.
Evid Based Complement Alternat Med. 2021 Apr 26;2021:5535727. doi: 10.1155/2021/5535727. eCollection 2021.
L. (Malvaceae) is a plant used in Burkina Faso for the treatment of various ailments including asthma. The aim of the study was to evaluate the pharmacological relaxant effect of the leafy stem extracts of and thereby verify claim of use in treating asthma. Aqueous decoction and hydroalcoholic extracts obtained from the powdered leafy stems were screened for the presence of some phytoconstituents. The relaxant effect of the two extracts was evaluated on acetylcholine- (ACh 10 M) and potassium chloride- (KCl 6 × 10 M) induced contractions on rat-isolated tracheal preparations. To examine whether the potassium (K) channels are involved in the relaxant effect, glibenclamide, an ATP-sensitive potassium channel inhibitor, was used. Moreover, to assess the safety of the extracts, acute oral toxicity was carried out on mice. The phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, steroids, triterpenoids, tannins, and coumarins in the hydroalcoholic extract. Tannins, steroids, triterpenoids, and coumarins were not detected in the aqueous decoction. With respective EC values of 1.517 ± 0.002 mg/mL and 1.433 ± 0.001 mg/mL on ACh-and KCl-provoked contractions, the hydroalcoholic extract was found more potent in relaxing the isolated rat tracheal preparations compared to the aqueous decoction. In the presence of glibenclamide, the relaxant effect of the hydroalcoholic extract (EC = 0.191 ± 0.002 mg/mL) increased and was higher than that of the aqueous decoction. At dose of 5000 mg/kg of body weight, the extracts did not produce deaths or any significant changes in the general behavior of mice. The results suggest that different mechanisms including modulation of calcium and potassium channels, particularly the ATP-sensitive K channels, could be involved in the relaxation effect. These findings could justify the traditional use of in the management of asthma.
[植物名称]（锦葵科）是布基纳法索用于治疗包括哮喘在内的各种疾病的一种植物。本研究的目的是评估[植物名称]带叶茎提取物的药理松弛作用，从而验证其用于治疗哮喘的说法。对从带叶茎粉末中获得的水煎剂和水醇提取物进行了一些植物成分的筛选。在大鼠离体气管制备物上，评估了这两种提取物对乙酰胆碱（ACh 10 μM）和氯化钾（KCl 6×10⁻² M）诱导的收缩的松弛作用。为了检查钾（K）通道是否参与松弛作用，使用了格列本脲，一种ATP敏感性钾通道抑制剂。此外，为了评估提取物的安全性，对小鼠进行了急性经口毒性试验。植物化学筛选显示水醇提取物中存在生物碱、黄酮类、皂苷、甾体、三萜类、单宁和香豆素。水煎剂中未检测到单宁、甾体、三萜类和香豆素。在ACh和KCl诱发的收缩上，水醇提取物的EC₅₀值分别为1.517±0.002 mg/mL和1.433±0.001 mg/mL，与水煎剂相比，水醇提取物在松弛离体大鼠气管制备物方面更有效。在格列本脲存在的情况下，水醇提取物的松弛作用（EC₅₀ = 0.191±0.002 mg/mL）增强，且高于水煎剂。在5000 mg/kg体重剂量下，提取物未导致小鼠死亡或一般行为出现任何显著变化。结果表明，包括钙通道和钾通道（特别是ATP敏感性钾通道）的调节在内的不同机制可能参与了松弛作用。这些发现可以证明[植物名称]在哮喘治疗中的传统用途是合理的。
