Nikzamir Abdolrahim, Rezaei-Tavirani Mostafa, Razzaghi Zahra, Rostami-Nejad Mohammad, Hamdieh Mostafa, Arjmand Babak
Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2020 Fall;11(Suppl 1):S80-S84. doi: 10.34172/jlms.2020.S13. Epub 2020 Dec 30.
Gamma radiation is accompanied by prominent biological effects and damages. Cell proliferation and tumorigenesis are highlighted as the main resulted effects of gamma radiation on cultured cells. This study aims to assess the dysregulated mode of gene function after gamma radiation in human Jurkat cells. Six gene expression profiles from Gene Expression Omnibus (GEO) were analyzed by GEO2R to find the significant differentially expressed genes (DEGs) via gamma radiation. Action map analysis was applied to screen the query DEGs. Among 108 study genes, 20 critical DEGs including AURKA, AURKB, BORA, CCNB1, CCNB2, CCNF, CDC20, CDCA8, CENPA, CENPE, CENPF, KIF18A, KIF20A, KIF23, BUB1, DLGAP5, ECT2, PLK1, SGO2, and TPX2 were introduced as down-regulated genes by the gamma ray. Activators of the introduced critical genes may be the cell protector against gamma radiation.
γ辐射伴随着显著的生物学效应和损伤。细胞增殖和肿瘤发生被视为γ辐射对培养细胞的主要影响结果。本研究旨在评估γ辐射后人Jurkat细胞中基因功能的失调模式。通过GEO2R分析了来自基因表达综合数据库(GEO)的六个基因表达谱,以找出经γ辐射后显著差异表达的基因(DEGs)。应用作用图谱分析来筛选查询的DEGs。在108个研究基因中,包括极光激酶A(AURKA)、极光激酶B(AURKB)、硼调节蛋白(BORA)、细胞周期蛋白B1(CCNB1)、细胞周期蛋白B2(CCNB2)、细胞周期蛋白F(CCNF)、细胞分裂周期蛋白20(CDC20)、细胞分裂周期相关蛋白8(CDCA8)、着丝粒蛋白A(CENPA)、着丝粒蛋白E(CENPE)、着丝粒蛋白F(CENPF)、驱动蛋白家族成员18A(KIF18A)、驱动蛋白家族成员20A(KIF20A)、驱动蛋白家族成员23(KIF23)、纺锤体检查点蛋白BUB1(BUB1)、Dlg家族结合蛋白5(DLGAP5)、上皮细胞转化蛋白2(ECT2)、丝氨酸/苏氨酸蛋白激酶1(PLK1)、减数分裂重组蛋白SGO2(SGO2)和微管蛋白聚合促进蛋白2(TPX2)在内的20个关键DEGs被确定为受γ射线下调的基因。所引入关键基因的激活剂可能是细胞抵抗γ辐射的保护因子。