Vafaee Reza, Nikzamir Abdolrahim, Razzaghi Mohhamadreza, Rezaei Tavirani Sina, Ahmadzadeh Alireza, Emamhadi MohammadAli
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2020 Fall;11(Suppl 1):S101-S106. doi: 10.34172/jlms.2020.S16. Epub 2020 Dec 30.
Genomics and bioinformatics are useful methods for exploring unclear aspects of radiation effects on biological systems. Many radiation-induced alterations in irradiated samples are post-radiation time-dependent. This study aims to evaluate the post-irradiation effects of the gamma ray on human Jurkat cells. Gene expression profiles of the samples harvested 6 and 24 hours after radiation to find the critical differential expressed genes and the related pathways. Samples are provided from Gene Expression Omnibus (GEO) and analyzed by ClueGO. Twnety-nine critical genes were determined as the important affected genes and 7 classes of related pathways were introduced. CCNE2, PSMD11, CDC25C, ANAPC1, PLK1, AURKA, and CCNB1 that were associated with more than 6 pathways were related to one of the determined pathway groups. Cell protecting pathways were associated with the genes (HSPA5, HSPA8, HSP90B1, HMMR, CEBPB, RXRA, and PSMD11) which were related to the minimum numbers of pathways. The finding of this study corresponds to repair processes which depend on post-radiation time. It seems these sets of genes are suitable candidates for further investigation.
基因组学和生物信息学是探索辐射对生物系统影响的不明方面的有用方法。辐照样品中许多辐射诱导的变化是辐射后时间依赖性的。本研究旨在评估γ射线对人Jurkat细胞的辐照后效应。对辐射后6小时和24小时收获的样品进行基因表达谱分析，以找出关键的差异表达基因和相关途径。样品来自基因表达综合数据库（GEO）并通过ClueGO进行分析。确定了29个关键基因作为重要的受影响基因，并介绍了7类相关途径。与6条以上途径相关的CCNE2、PSMD11、CDC25C、ANAPC1、PLK1、AURKA和CCNB1与所确定的途径组之一相关。细胞保护途径与涉及途径数量最少的基因（HSPA5、HSPA8、HSP90B1、HMMR、CEBPB、RXRA和PSMD11）相关。本研究的发现与依赖于辐射后时间的修复过程一致。这些基因集似乎是进一步研究的合适候选对象。
