Wu Shulian, Okada Ryuhei, Liu Yi, Fang Yuhong, Yan Feng, Wang Chen, Li Hui, Kobayashi Hisataka, Chen Yu, Tang Qinggong
College of Photonic and Electronic Engineering, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, 350007, China.
Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Biomed Opt Express. 2021 Mar 2;12(4):1804-1820. doi: 10.1364/BOE.419163. eCollection 2021 Apr 1.
Near-infrared (NIR) photoimmunotherapy (NIR-PIT) is an emerging cancer therapy based on a monoclonal antibody and phthalocyanine dye conjugate. Direct tumor necrosis and immunogenic cell death occur during NIR irradiation. However, the alteration of tumor blood vessels and blood volume inside the blood vessels induced by the NIR-PIT process is still unknown. In our study, a speckle variance (SV) algorithm combined with optical coherence tomography (OCT) technology was applied to monitor the change of blood vessels and the alterations of the blood volume inside the blood vessels during and after NIR-PIT treatment. Vascular density and the measurable diameter of the lumen in the blood vessel (the diameter of the region filled with blood) were extracted for quantitively uncovering the alterations of blood vessels and blood volume induced by NIR-PIT treatment. The results indicate that both the density and the diameter of the lumen in the blood vessels decrease during the NIR-PIT process, while histological results indicated the blood vessels were dilated. The increase of permeability of blood vessels could lead to the increase of the blood pool volume within the tumor (shown in histology) and results in the decrease of free-moving red blood cells inside the blood vessels (shown in SV-OCT).
近红外（NIR）光免疫疗法（NIR-PIT）是一种基于单克隆抗体与酞菁染料偶联物的新兴癌症治疗方法。在近红外照射过程中会发生直接肿瘤坏死和免疫原性细胞死亡。然而，NIR-PIT过程引起的肿瘤血管变化以及血管内血容量的改变仍不清楚。在我们的研究中，将散斑方差（SV）算法与光学相干断层扫描（OCT）技术相结合，用于监测NIR-PIT治疗期间及之后血管的变化以及血管内血容量的改变。提取血管密度和血管内腔的可测量直径（充满血液区域的直径），以定量揭示NIR-PIT治疗引起的血管和血容量变化。结果表明，在NIR-PIT过程中血管密度和血管内腔直径均减小，而组织学结果显示血管扩张。血管通透性增加会导致肿瘤内血池体积增加（组织学显示），并导致血管内自由移动红细胞减少（SV-OCT显示）。
