Damrongsri Damrong, Nowwarote Nunthawan, Sonpoung Opor, Photichailert Suphalak, Osathanon Thanaphum
Dental Stem Cell Biology Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand.
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand.
J Oral Biol Craniofac Res. 2021 Jul-Sep;11(3):379-385. doi: 10.1016/j.jobcr.2021.04.004. Epub 2021 Apr 20.
Although dental pulp and apical papilla are originated from neural crest cells, these tissues exhibit distinct characteristics. Notch signaling is one of the known signaling pathways regulating stemness and behaviors of stem cells. The aim of this study was to examine Notch signaling related gene expression profile comparing between coronal pulp tissues and apical pulp complex. Results demonstrated that coronal pulp tissue had higher expression levels of various genes in Notch pathway. However, , , , and mRNA levels were significantly lower in coronal pulp tissue than those of apical pulp complex. Furthermore, dental pulp stem cells (DPSCs) and stem cells isolated from apical papilla (SCAPs) were isolated and characterized. These two cell types exhibited similar mesenchymal stem cell surface markers. DPSCs expressed higher mRNA levels of and . In addition, SCAPs demonstrated higher colony formation and cell proliferation than DPSCs. In summary, cells and tissues from dental pulp and apical papilla exhibited the distinct gene expression profile of Notch related genes. This could be of one the signaling participated in control of DPSCs and SCAPs cells behaviors.
尽管牙髓和根尖乳头起源于神经嵴细胞，但这些组织表现出不同的特征。Notch信号通路是已知的调节干细胞干性和行为的信号通路之一。本研究的目的是比较冠髓组织和根尖牙髓复合体中Notch信号相关基因的表达谱。结果表明，冠髓组织中Notch通路中各种基因的表达水平较高。然而， 、 、 和 的mRNA水平在冠髓组织中显著低于根尖牙髓复合体。此外，分离并鉴定了牙髓干细胞（DPSCs）和从根尖乳头分离的干细胞（SCAPs）。这两种细胞类型表现出相似的间充质干细胞表面标志物。DPSCs表达的 和 的mRNA水平较高。此外，SCAPs比DPSCs表现出更高的集落形成和细胞增殖能力。总之，牙髓和根尖乳头的细胞和组织表现出Notch相关基因的不同表达谱。这可能是参与控制DPSCs和SCAPs细胞行为的信号之一。
