Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Republic of Korea.
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Republic of Korea; Oral Science Research Center, College of Dentistry, Yonsei University, Seoul, Republic of Korea.
J Endod. 2018 Jan;44(1):87-92.e1. doi: 10.1016/j.joen.2017.08.018. Epub 2017 Nov 1.
The aim of this study was to measure and compare the expression levels of cytokines from developing apical complex cells (DACCs) and dental pulp stem cells (DPSCs) of the immature tooth.
DPSC-conditioned medium (CM) and DACCs-CM were obtained from human young teeth, and 174 cytokines secreted from each CM were identified and compared. A cytokine membrane array and enzyme-linked immunosorbent assay were used to measure and compare the expression levels of the cytokines. Immunocytochemistry targeting insulin-like growth factor-1 and neurotrophin-3 was additionally performed.
There were statistically significant differences in the expression levels of 25 cytokines: 22 and 3 were expressed more strongly in DPSCs-CM and DACCs-CM, respectively. Odontoblast differentiation-related cytokines were more strongly expressed in DPSCs-CM, while cell-proliferation-related cytokines were more strongly expressed in DACCs-CM. Proinflammatory and anti-inflammatory cytokines were predominantly expressed in DPSCs-CM and DACCs-CM, respectively.
DPSCs may exert a stronger paracrine effect than DACCs on regeneration of the dentin-pulp complex, in terms of odontoblast differentiation.
本研究旨在测量和比较未成熟牙中根尖复合体细胞（DACC）和牙髓干细胞（DPSC）的细胞因子表达水平。
从人类年轻牙齿中获得 DPSC 条件培养基（CM）和 DACC-CM，并鉴定和比较每种 CM 分泌的 174 种细胞因子。使用细胞因子膜阵列和酶联免疫吸附试验测量和比较细胞因子的表达水平。此外，还进行了针对胰岛素样生长因子-1 和神经营养因子-3 的免疫细胞化学。
在 25 种细胞因子的表达水平上存在统计学显著差异：22 种和 3 种在 DPSC-CM 和 DACC-CM 中的表达分别更强。牙本质-牙髓复合体再生相关的成牙本质细胞分化相关细胞因子在 DPSC-CM 中表达更强，而细胞增殖相关细胞因子在 DACC-CM 中表达更强。促炎和抗炎细胞因子分别主要在 DPSC-CM 和 DACC-CM 中表达。
就牙本质-牙髓复合体的成牙本质细胞分化而言，DPSC 对牙本质-牙髓复合体的再生可能比 DACC 发挥更强的旁分泌作用。
