Youssef Mervat M, El-Mansy Merhan N, El-Borady Ola M, Hegazy Enas M
Faculty of Dentistry, Suez Canal University, Kilo 4.5 Ring Road., Ismailia, Egypt.
Institute of Nanoscience and Nanotechnology, Kafrelsheikh University, 33516, Kafrelsheikh, Egypt.
J Oral Biol Craniofac Res. 2021 Jul-Sep;11(3):386-392. doi: 10.1016/j.jobcr.2021.04.002. Epub 2021 Apr 14.
This study aimed to evaluate the potential cytotoxic effect of oral administration of silver nanoparticles (Ag-NPs) on adult albino rats' pulp tissue; due to the enormous uses of Ag-NPs in the medical and dental field. The Ag-NPs were synthesized via the green process using peels of pomegranate extract. The pomegranate-mediated Ag-NPs were subjected to morphological and spectral analysis through ultraviolet visible absorption spectra, transmission electron microscopy, Fourier transforms infrared, Zeta-potential measurements, and energy dispersive X-ray spectroscopy. The structural and morphological characterization techniques confirmed the proper synthesis of biosynthesized Ag-NPs with a size around 20 nm and the surface plasmon resonance peak within 400-450 nm. The oral cytotoxic effect of Ag-NPs was assessed through detecting the histological (hematoxylin & eosin, Masson's trichrome) and immunohistochemical (vascular endothelial growth factor (VEGF), Caspase-3 proteins) variations. The data was analyzed statistically through using the SPSS software. Dental pulp tissues of albino rats-treated with Ag-NPs revealed that most of the odontoblasts with marked hydropic degeneration, vacuolization of their cytoplasm, loss of organization and apoptosis. Marked vasodilatation and cognition of blood vessels were detected. There was weak to moderate positive reactivity to Masson's trichrome stain. There was statistically significant decrease in the expression of VEGF in the treated group and highly statistically significant increase in the expression of Caspase-3 in comparison with the control group.
Oral administration of Ag-NPs induced size and dose-dependent structural changes in the pulp tissue of adult male albino rats.
本研究旨在评估口服银纳米颗粒（Ag-NPs）对成年白化大鼠牙髓组织的潜在细胞毒性作用；因为Ag-NPs在医学和牙科领域有大量应用。通过使用石榴皮提取物的绿色工艺合成Ag-NPs。通过紫外可见吸收光谱、透射电子显微镜、傅里叶变换红外光谱、Zeta电位测量和能量色散X射线光谱对石榴介导的Ag-NPs进行形态和光谱分析。结构和形态表征技术证实了生物合成的Ag-NPs合成适当，尺寸约为20纳米，表面等离子体共振峰在400 - 450纳米范围内。通过检测组织学（苏木精和伊红染色、Masson三色染色）和免疫组织化学（血管内皮生长因子（VEGF）、半胱天冬酶-3蛋白）变化来评估Ag-NPs的口服细胞毒性作用。使用SPSS软件对数据进行统计学分析。用Ag-NPs处理的白化大鼠牙髓组织显示，大多数成牙本质细胞有明显的水样变性、细胞质空泡化、组织结构丧失和凋亡。检测到明显的血管扩张和血管充血。对Masson三色染色有弱至中度阳性反应。与对照组相比，治疗组VEGF表达有统计学意义的降低，半胱天冬酶-3表达有高度统计学意义的增加。
口服Ag-NPs可诱导成年雄性白化大鼠牙髓组织发生大小和剂量依赖性的结构变化。
