Mohammad Yousof Shimaa, Erfan Horeya, Mohamed Hosny Marwa, Shehata Shaimaa A, El-Sayed Karima
Department of Medical Physiology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Department of Histology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Saudi J Biol Sci. 2022 May;29(5):3890-3898. doi: 10.1016/j.sjbs.2022.02.054. Epub 2022 Mar 11.
Products containing Silver nanoparticles (Ag NPs) are becoming vastly used in our daily life. The widespread increased introduction of Ag NPs in many aspects of life has raised researchers' concerns regarding their safety and toxicity for biological and environmental life in the past few years. The current study aimed to explore the subsequent effects of Ag NPs withdrawal, following short-term oral administration. Eighteen rats were assigned randomly into three groups (control group "1" and AG NPs treated groups "2" and "3"; 6 animals each). The control group received normal food and tap water while groups 2 & 3 received 0.5 ml of a solution containing 25 ppm Ag NPs for 14 days. Group 2 rats were sacrificed on day 14 whereas group 3 was left for another 14 days of particle cessation followed by euthanasia on day 28. Functional assessment was done by liver enzyme assays, hydrogen peroxide activity, hepatic expression, and P53 immunoreactivity. Hepatic tissue structural assessment was done via hematoxylin and eosin, periodic acid-Schiff as well as Masson's trichrome stains. The results revealed a significant elevation of Hydrogen peroxide in group 2 only compared to the control group. Hepatic and liver enzymes were both insignificantly affected. Structural abnormalities and enhanced apoptosis in hepatic tissue were found 14 days after ceasing the nanoparticles. In conclusion: Structural and functional insults following Ag NPs oral administration continues after particle withdrawal, and interestingly they do not necessitate apparent reflection on liver enzyme assays.
含有银纳米颗粒（Ag NPs）的产品在我们日常生活中的使用正变得越来越广泛。在过去几年里，Ag NPs在生活诸多方面的广泛引入增加，引发了研究人员对其对生物和环境生命安全及毒性的担忧。本研究旨在探究短期口服给药后停止使用Ag NPs的后续影响。18只大鼠被随机分为三组（对照组“1”和Ag NPs处理组“2”和“3”；每组6只动物）。对照组给予正常食物和自来水，而第2组和第3组连续14天接受0.5毫升含25 ppm Ag NPs的溶液。第2组大鼠在第14天处死，而第3组在停止给予颗粒后再饲养14天，然后在第28天实施安乐死。通过肝酶测定、过氧化氢活性、肝脏表达和P53免疫反应性进行功能评估。通过苏木精和伊红染色、过碘酸 - 希夫染色以及马松三色染色进行肝组织结构评估。结果显示，仅第2组的过氧化氢水平与对照组相比有显著升高。肝脏表达和肝酶均未受到显著影响。停止给予纳米颗粒14天后，发现肝组织存在结构异常和凋亡增加。总之：口服Ag NPs后，在停止给予颗粒后，结构和功能损伤仍会持续，有趣的是，它们不一定会在肝酶测定中表现出明显反映。
