Hernandez Luciana, Bottini Enriqueta, Cadona Jimena, Cacciato Claudio, Monteavaro Cristina, Bustamante Ana, Sanso Andrea Mariel
Laboratorio de Inmunoquímica y Biotecnología, CIVETAN (CONICET), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Tandil, Argentina.
Laboratorio de Microbiología Clínica y Experimental, CIVETAN (CONICET), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Tandil, Argentina.
Front Cell Infect Microbiol. 2021 Apr 30;11:647324. doi: 10.3389/fcimb.2021.647324. eCollection 2021.
is a pathogen-associated to bovine mastitis, a health disorder responsible for significant economic losses in the dairy industry. Antimicrobial therapy remains the main strategy for the control of this bacterium in dairy herds and human In order to get insight on molecular characteristics of strains circulating among Argentinean cattle with mastitis, we received 1500 samples from 56 dairy farms between 2016 and 2019. We recovered 56 isolates and characterized them in relation to serotypes, virulence genes, and antimicrobial susceptibility. Serotypes III and II were the most prevalent ones (46% and 41%, respectively), followed by Ia (7%). In relation to the 13 virulence genes screened in this study, the genes , and PI-2b were present in all the isolates, meanwhile, , , and were detected in different frequencies, 36%, 96%, and 59%, respectively. On the other hand, , PI-1, PI-2a, and genes could not be detected in any of the isolates. Disk diffusion method against a panel of eight antimicrobial agents showed an important number of strains resistant simultaneously to five antibiotics. We also detected several resistance-encoding genes, , and (9%, 50%, 32%, 32%, and 5%, respectively). The results here presented are the first molecular data on isolates causing bovine mastitis in Argentina and provide a foundation for the development of diagnostic, prophylactic, and therapeutic methods, including the perspective of a vaccine.
是一种与牛乳腺炎相关的病原体，牛乳腺炎是一种健康紊乱疾病，给乳制品行业造成重大经济损失。抗菌治疗仍然是控制奶牛场和人类中这种细菌的主要策略。为了深入了解在阿根廷患乳腺炎的牛群中传播的菌株的分子特征，我们在2016年至2019年期间从56个奶牛场收到了1500份样本。我们分离出56株菌株，并对其血清型、毒力基因和抗菌药敏性进行了鉴定。血清型III和II最为普遍（分别为46%和41%），其次是Ia（7%）。关于本研究中筛选的13个毒力基因，所有分离株中均存在基因、和PI-2b，同时，、和以不同频率被检测到，分别为36%、96%和59%。另一方面，在任何分离株中均未检测到、PI-1、PI-2a和基因。针对一组八种抗菌剂的纸片扩散法显示，有相当数量的菌株同时对五种抗生素耐药。我们还检测到了几种耐药编码基因，、和（分别为9%、50%、32%、32%和5%）。这里呈现的结果是关于阿根廷引起牛乳腺炎的分离株的首批分子数据，为包括疫苗研发前景在内的诊断、预防和治疗方法的开发提供了基础。
