Hassan Jayedul, Bag Abdus Sattar, Karmakar Susmita, Utsho Kishor Sosmith, Ali Wohab, Kabir Ajran, Rahman Tanvir
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
J Adv Vet Anim Res. 2025 Mar 24;12(1):80-89. doi: 10.5455/javar.2025.l874. eCollection 2025 Mar.
The goal of this study is to describe the genome of that was found in clinical mastitis in cattle in Bangladesh. This work will show how strong the bacteria are and how important they are for public health.
Whole genome sequencing (WGS) was performed using the Illumina MiSeq platform, followed by comprehensive analysis with various bioinformatic tools to identify key genomic features.
WGS revealed that the isolates are closely related, belonging to sequence type ST4, a rare type previously identified in both human and animal hosts. The isolates possess 44 virulence-related genes linked to adherence, capsule biogenesis, enzyme production, immunoreactive antigens, protease, and cytolysin production. They also carry two pilus islands (PIs), PI-1 and PI-2b, which are often associated with invasive diseases. PI-2b proteins are key targets for vaccine development against Group B Streptococcus (GBS). The isolates belong to serotype Ia and carry the gbs2018-2 variant, indicating their adaptability to a wide range of hosts, including humans and animals. These virulence factors are critical for understanding s pathogenicity and developing vaccines against its infections. Additionally, the isolates harbor antimicrobial resistance genes conferring resistance to glycopeptides (, ), macrolides (), peptides (), penicillins and -lactams (), and aminoglycosides. Source tracking via the BacWGSTdb website identified these isolates as closely related to human pathogens, indicating their zoonotic potential.
These results suggest that could be a zoonotic pathogen. This highlights the need for ongoing genomic surveillance to fully understand how it causes disease and come up with effective ways to control it.
本研究的目的是描述在孟加拉国牛临床乳腺炎中发现的[细菌名称未给出]的基因组。这项工作将展示该细菌的强大程度以及它们对公共卫生的重要性。
使用Illumina MiSeq平台进行全基因组测序（WGS），随后使用各种生物信息学工具进行综合分析，以识别关键的基因组特征。
WGS显示这些分离株密切相关，属于序列类型ST4，这是一种先前在人类和动物宿主中均已鉴定出的罕见类型。这些分离株拥有44个与毒力相关的基因，这些基因与黏附、荚膜生物合成、酶产生、免疫反应性抗原、蛋白酶和细胞溶素产生有关。它们还携带两个菌毛岛（PI），PI-1和PI-2b，这通常与侵袭性疾病有关。PI-2b蛋白是针对B组链球菌（GBS）疫苗开发的关键靶点。这些分离株属于血清型Ia，并携带gbs2018-2变体，表明它们对包括人类和动物在内的广泛宿主具有适应性。这些毒力因子对于理解其致病性和开发针对其感染的疫苗至关重要。此外，这些分离株含有赋予对糖肽（[具体药物未给出]）、大环内酯类（[具体药物未给出]）、肽类（[具体药物未给出]）、青霉素和β-内酰胺类（[具体药物未给出]）以及氨基糖苷类耐药的抗菌抗性基因。通过BacWGSTdb网站进行的来源追踪确定这些分离株与人类病原体密切相关，表明它们具有人畜共患病潜力。
这些结果表明[细菌名称未给出]可能是人畜共患病病原体。这凸显了持续进行基因组监测以充分了解其致病方式并想出有效控制方法的必要性。
