Latief Miftahul Akhyar, Chikama Taiichiro, Ko Ji-Ae, Kiuchi Yoshiaki, Sakaguchi Takemasa, Obana Akira
Department of Ophthalmology and Visual Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan ; Medical Faculty, Muhammadiyah University, Makassar, South Sulawesi, Indonesia ; Department of Ophthalmology, Hasanuddin University, Makassar, South Sulawesi, Indonesia.
Department of Ophthalmology and Visual Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
Mol Vis. 2015 May 2;21:532-7. eCollection 2015.
To evaluate the efficacy of photodynamic antimicrobial chemotherapy (PACT) with the new porphyrin derivative TONS 504 and a light-emitting diode (LED) against acyclovir (ACV)-sensitive and -resistant herpes simplex virus type 1 (HSV-1).
Human FL cells infected with the viral strains were subjected to PACT with TONS 504 at various concentrations (0.01 to 10 mg/l) and irradiation at various light energies (10 to 30 J/cm(2)) and were then incubated for 24 h before analysis.
Immunocytofluorescence analysis with antibodies to HSV-1 revealed that PACT eliminated HSV-1 and ACV-resistant HSV-1 in a manner dependent on the TONS 504 concentration and light energy. Complete eradication of both viruses was apparent at a TONS 504 concentration of 10 mg/l and light energy of 10 to 30 J/cm(2) as well as at a TONS 504 concentration of 1 mg/l and light energy of 20 or 30 J/cm(2). No antiviral effect was apparent with TONS 504 in the absence of irradiation or with irradiation in the absence of TONS 504. Staining of cell nuclei with 4', 6-diamidino-2-phenylindole revealed no apparent cytotoxicity of the PACT system, a finding that was confirmed by the system's failure to induce the release of lactate dehydrogenase from the host cells.
We conclude that our PACT system based on TONS 504 and an LED is effective for eliminating HSV-1 and ACV-resistant HSV-1 without a harmful effect on host cells.
评估新型卟啉衍生物TONS 504联合发光二极管（LED）进行光动力抗菌化学疗法（PACT）对阿昔洛韦（ACV）敏感及耐药的1型单纯疱疹病毒（HSV-1）的疗效。
用不同浓度（0.01至10 mg/l）的TONS 504对感染病毒株的人FL细胞进行PACT处理，并给予不同光能（10至30 J/cm²）照射，然后孵育24小时后进行分析。
用抗HSV-1抗体进行免疫细胞荧光分析显示，PACT以依赖于TONS 504浓度和光能的方式消除HSV-1和ACV耐药的HSV-1。在TONS 504浓度为10 mg/l且光能为10至30 J/cm²以及TONS 504浓度为1 mg/l且光能为20或30 J/cm²时，两种病毒均被完全清除。在无照射时TONS 504或无TONS 504时照射均未显示出抗病毒效果。用4',6-二脒基-2-苯基吲哚对细胞核进行染色显示PACT系统无明显细胞毒性，这一结果通过该系统未诱导宿主细胞释放乳酸脱氢酶得到证实。
我们得出结论，基于TONS 504和LED的PACT系统可有效消除HSV-1和ACV耐药的HSV-1，且对宿主细胞无有害影响。
