Gao Jucan, Jiang Lihong, Lian Jiazhang
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 310027, China.
Synth Syst Biotechnol. 2021 May 3;6(2):110-119. doi: 10.1016/j.synbio.2021.04.005. eCollection 2021 Jun.
The methylotrophic yeast (a.k.a. ) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, has unique biological characteristics and its expression system has been well developed. With the advances in synthetic biology, more efforts have been devoted to developing into a chassis for the production of various high-value compounds, such as natural products. This review begins with the introduction of synthetic biology tools for the engineering of , including vectors, promoters, and terminators for heterologous gene expression as well as Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated System (CRISPR/Cas) for genome editing. This review is then followed by examples of the production of value-added natural products in metabolically engineered strains. Finally, challenges and outlooks in developing as a synthetic biology chassis are prospected.
甲基营养型酵母（又称 ）是工业生产重组蛋白最常用的宿主之一。作为一种非常规酵母， 具有独特的生物学特性，其表达系统也已得到充分发展。随着合成生物学的进步，人们投入了更多努力将 开发成用于生产各种高价值化合物（如天然产物）的底盘细胞。本综述首先介绍用于 工程改造的合成生物学工具，包括用于异源基因表达的载体、启动子和终止子，以及用于基因组编辑的成簇规律间隔短回文重复序列/CRISPR相关系统（CRISPR/Cas）。接着是代谢工程改造的 菌株生产增值天然产物的实例。最后，展望了将 开发成合成生物学底盘细胞所面临的挑战和前景。
