Zhang Yi, Li Chenglin, Zhu Qiuwen, Liang Renjie, Xie Chang, Zhang Shufang, Hong Yi, Ouyang Hongwei
Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Zhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China.
Bioact Mater. 2021 May 5;6(12):4447-4454. doi: 10.1016/j.bioactmat.2021.04.032. eCollection 2021 Dec.
Corneal injuries will cause corneal surface diseases that may lead to blindness in millions of people worldwide. There is a tremendous need for biomaterials that can promote corneal regeneration with practical feasibility. Here we demonstrate a strategy of a protein coating for corneal injury regeneration. We synthesize an o-nitrosobenzaldehyde group (NB)-modified gelatin (GelNB), which could adhere directly to the corneal surface with covalent bonding to form a thin molecular coating. The molecular coating could avoid rapid clearance and provide a favorable environment for cell migration, thereby effectively accelerating corneal repair and regeneration. The histological structure of the regenerated cornea is more similar to the native cornea. This molecular coating can be used conveniently as an eye drop solution, which makes it a promising strategy for corneal regeneration.
角膜损伤会引发角膜表面疾病，这可能导致全球数百万人失明。对于具有实际可行性的、能够促进角膜再生的生物材料有着巨大的需求。在此，我们展示了一种用于角膜损伤再生的蛋白质涂层策略。我们合成了一种邻亚硝基苯甲醛基团（NB）修饰的明胶（GelNB），它能够通过共价键直接附着在角膜表面，形成一层薄的分子涂层。该分子涂层能够避免快速清除，并为细胞迁移提供有利环境，从而有效加速角膜修复和再生。再生角膜的组织结构与天然角膜更为相似。这种分子涂层可以方便地制成滴眼液使用，这使其成为一种有前景的角膜再生策略。
