University/BHF Centre for Cardiovascular Science, University of Edinburgh, The Queen's Medical Research Institute, Edinburgh BioQuarter, 47 Little France Crescent, Edinburgh EH16 4TJ, UK.
Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh BioQuarter, 5 Little France Crescent, Edinburgh EH16 4UU, UK.
STAR Protoc. 2021 Apr 21;2(2):100493. doi: 10.1016/j.xpro.2021.100493. eCollection 2021 Jun 18.
This protocol describes the production of hepatocyte-like cells (HLCs) from human pluripotent stem cells and how to induce hepatic steatosis, a condition characterized by intracellular lipid accumulation. Following differentiation to an HLC phenotype, intracellular lipid accumulation is induced with a steatosis induction cocktail, allowing the user to examine the cellular processes that underpin hepatic steatosis. Furthermore, the renewable nature of our system, on a defined genetic background, permits in-depth mechanistic analysis, which may facilitate therapeutic target identification in the future. For complete details on the use and execution of this protocol, please refer to Sinton et al. (2021).
本方案描述了从人类多能干细胞生成肝样细胞（HLC）的方法，以及如何诱导肝脂肪变性，即细胞内脂质积累的一种病症。在分化为 HLC 表型后，用脂肪变性诱导混合物诱导细胞内脂质积累，从而可以检查支持肝脂肪变性的细胞过程。此外，在定义的遗传背景下，我们的可再生系统允许进行深入的机制分析，这有助于将来确定治疗靶点。如需详细了解本方案的使用和实施，请参考 Sinton 等人（2021 年）的文献。
