Centre for Regenerative Medicine, Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh BioQuarter, 5 Little France Drive, Edinburgh EH16 4UU, UK.
STAR Protoc. 2021 Apr 30;2(2):100502. doi: 10.1016/j.xpro.2021.100502. eCollection 2021 Jun 18.
This protocol describes how to produce human liver spheres from pluripotent stem cell-derived hepatic progenitors, endothelial cells, and hepatic stellate cells. Liver spheres form by self-assembly in microwells, generating up to 73 spheres per well of a 96-well plate. This process was automated using liquid handling and pipetting systems, permitting cost-effective scale-up and reducing sphere variability. In its current form, this system provides a powerful tool to generate human liver tissue for disease modeling and drug screening. For complete details on the use and execution of this protocol, please refer to Lucendo-Villarin et al. (2020) (https://doi.org/10.1088/1758-5090/abbdb2).
本方案描述了如何从多能干细胞来源的肝祖细胞、内皮细胞和肝星状细胞中产生人肝球体。肝球体在微井中通过自组装形成，每个 96 孔板的孔中可形成多达 73 个球体。该过程使用液体处理和移液系统自动化进行，允许经济高效地扩大规模并减少球体的变异性。在当前形式下，该系统为生成用于疾病建模和药物筛选的人肝组织提供了强大的工具。有关该方案的使用和执行的完整详细信息，请参阅 Lucendo-Villarin 等人（2020 年）（https://doi.org/10.1088/1758-5090/abbdb2）。
